从古代粪便中提取DNA。

Extraction of DNA from paleofeces.

作者信息

Kuch Melanie, Poinar Hendrik

机构信息

McMaster Ancient DNA Centre, McMaster University, Hamilton, ON, Canada.

出版信息

Methods Mol Biol. 2012;840:37-42. doi: 10.1007/978-1-61779-516-9_5.

DOI:10.1007/978-1-61779-516-9_5

PMID:22237519

Abstract

Paleofeces are the nonmineralized remains of dung from extant and extinct fauna. They represent a surprisingly large proportion of fossil remains recovered from cave sites across the world. Paleofeces contain the DNA of the defecator as well as the DNA of ingested plant and animal remains. To successfully extract DNA from paleofeces, a balance must be achieved between the minimization of DNA loss during extraction and the removal of coeluates that would otherwise inhibit the Taq DNA polymerase during downstream applications. Here we present a simplified version of a protocol to extract DNA from paleofecal remains.

摘要

古粪便即现存和已灭绝动物粪便的非矿化残留物。它们在世界各地洞穴遗址出土的化石残骸中所占比例大得出奇。古粪便含有排便者的DNA以及摄入的动植物残骸的DNA。要成功从古粪便中提取DNA，必须在尽量减少提取过程中的DNA损失与去除否则会在下游应用中抑制Taq DNA聚合酶的共洗脱物之间取得平衡。在此，我们展示了一种从古粪便残骸中提取DNA的简化方案。