具有广泛底物范围的嵌合自给 P450cam-RhFRed 生物催化剂。
Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope.
机构信息
School of Chemistry & Manchester Interdisciplinary Biocentre (MIB), University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
出版信息
Beilstein J Org Chem. 2011;7:1494-8. doi: 10.3762/bjoc.7.173. Epub 2011 Nov 2.
Abstract
A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities.
摘要
开发了一种高通量筛选方案，用于评估嵌合、自给自足的 P450 生物催化剂及其突变体对一系列底物的活性，从而鉴定出许多新的生物氧化活性。
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