El'chaninov V V, Fedorovich I B
Biokhimiia. 1990 Jul;55(7):1304-8.
The kinetics of individual crystalline SH-group modification by DTNB were studied. According to the rates of their interaction with the modifier, the thiol groups in the native protein molecule can be classified as free, accessible, weakly modified and "masked" ones. Denaturation by the detergent (CTAB) caused an increase in the SH-group modification rate. In this case the SH-groups were modified as free and accessible ones. Illumination with UV-light resulted in a decrease in the number of SH-groups, opening of "masked" SH-groups in almost all crystallines except for alpha-crystalline, and essential changes in the SH-group modification rate.
研究了二硫代硝基苯甲酸（DTNB）对单个结晶性巯基（SH-基团）的修饰动力学。根据其与修饰剂相互作用的速率，天然蛋白质分子中的巯基可分为游离的、可及的、弱修饰的和“被掩盖的”巯基。去污剂（十六烷基三甲基溴化铵，CTAB）引起的变性导致SH-基团修饰速率增加。在这种情况下，SH-基团作为游离的和可及的巯基被修饰。紫外线照射导致SH-基团数量减少，除α-晶状体蛋白外，几乎所有晶状体中的“被掩盖的”SH-基团都被打开，并且SH-基团修饰速率发生了显著变化。
