[一种用于在聚丙烯酰胺凝胶中制备经博尔顿-亨特试剂标记的皮摩尔量蛋白质的肽图的方法]
[A method for producing peptide maps of picomolar quantities of protein labelled with the Bolton-Hunter reagent in polyacrylamide gel].
作者信息
Kolomiĭtseva G Ia, Kurochkina L P, Nikitina I V, Moiseeva T I
出版信息
Nauchnye Doki Vyss Shkoly Biol Nauki. 1990(8):152-5.
PMID:2223906
Abstract
A method of radioiodination to high specific activity by Bolton Hunter reagent of fixed and stained histones in polyacrylamide gel is described. The comparative peptide maps of the histones H1 and H3, labelled in solution and the gel are presented.
摘要
本文描述了一种使用博尔顿·亨特试剂对聚丙烯酰胺凝胶中固定和染色的组蛋白进行高比活放射性碘化的方法。给出了在溶液中和凝胶中标记的组蛋白H1和H3的比较肽图。
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