Feldman A M, Epstein M H, Brusilow S W
Brain Res. 1979 May 5;167(1):119-28. doi: 10.1016/0006-8993(79)90267-1.
The present study was performed to measure the effects of cholera toxin (CT) and prostaglandins (PG) on choroid plexus cyclic AMP accumulation. Choroid plexuses, isolated from Sprague-Dawley rats, were incubated in Krebs-Ringer bicarbonate buffer containing either CT (10 microgram/ml), heat inactivated CT, prostaglandins, or appropriate controls. After a minimum incubation period of 60 min with CT, cyclic AMP accumulation (pmol/mg protein) in treated incubates (58.10 +/- 6.43, n = 6) was 4 times that in controls (14.-5 +/- 1.4, n = 6). This large increase was only seen when theophylline (10 mM) was in the incubation mixture 3--10 min prior to the end of the incubation period. The increase in cyclic AMP accumulation was dose responsive and was irreversible after 5 min of incubation. Of the 5 prostaglandins studied, only PGE2 resulted in an increase in cyclic AMP accumulation. Cyclic AMP levels were 29.64 +/- 2.5 in controls and 57.57 +/- 3.5 in plexus which had been incubated with PGE2 (20 microgram/ml) for 1 min, and this increase was dose responsive.
本研究旨在测定霍乱毒素（CT）和前列腺素（PG）对脉络丛环磷酸腺苷（cAMP）积聚的影响。从Sprague-Dawley大鼠分离出的脉络丛，在含有CT（10微克/毫升）、热灭活CT、前列腺素或适当对照的 Krebs-Ringer 碳酸氢盐缓冲液中孵育。在用CT孵育至少60分钟后，处理过的孵育物（58.10±6.43，n = 6）中的cAMP积聚（皮摩尔/毫克蛋白质）是对照（14.5±1.4，n = 6）中的4倍。只有当在孵育期结束前3 - 10分钟，孵育混合物中含有茶碱（10毫摩尔）时，才会出现这种大幅增加。cAMP积聚的增加具有剂量反应性，并且在孵育5分钟后是不可逆的。在所研究的5种前列腺素中，只有PGE2导致cAMP积聚增加。对照中的cAMP水平为29.64±2.5，与PGE2（20微克/毫升）孵育1分钟的脉络丛中的cAMP水平为57.57±3.5，这种增加具有剂量反应性。
