State Key Laboratory of Marine Environmental Science, Marine Biodiversity and Global Change Center, College of Oceanography and Environmental Sciences, Xiamen University, Xiamen, 361005, China.
J Eukaryot Microbiol. 2012 Mar-Apr;59(2):157-62. doi: 10.1111/j.1550-7408.2011.00603.x. Epub 2012 Jan 12.
We used inter-simple sequence repeat fingerprinting to analyze the genetic structure of 16 populations of Stentor coeruleus from three lakes and three ponds in China. Using 14 polymorphic primers, a total of 99 discernible DNA fragments were detected, among which 76 (76.77%) were polymorphic, indicating median genetic diversity in these populations. Further, both Nei's gene diversity (h) and Shannon's information index (I) between the different populations revealed a median genetic diversity. At the same time, gene flow was interpreted to be low. The main factors responsible for the median level of diversity and low gene flow within populations are probably due to a low frequency of sexual recombinations. Analysis of molecular variance showed that there was high genetic differentiation among the five water bodies. Both cluster analysis and a nonmetric multidimensional scaling analysis suggested that genotypes isolated from the same locations displayed a higher genetic similarity than those from different ones, separating populations into subgroups according to their geographical locations. However, there is a weak positive correlation between the genetic distance and geographical distance.
我们使用简单序列重复指纹分析技术，对来自中国三个湖泊和三个池塘的 16 个 Stentor coeruleus 种群的遗传结构进行了分析。使用 14 个多态性引物，共检测到 99 个可识别的 DNA 片段，其中 76 个（76.77%）是多态性的，表明这些种群具有中等的遗传多样性。此外，不同种群之间的 Nei's 基因多样性（h）和 Shannon's 信息指数（I）都显示出中等的遗传多样性。同时，基因流被解释为低水平的。导致种群内中等水平多样性和低基因流的主要因素可能是由于性重组的频率较低。分子方差分析表明，五个水体之间存在高度的遗传分化。聚类分析和非度量多维尺度分析都表明，来自同一地点的基因型比来自不同地点的基因型具有更高的遗传相似性，根据地理位置将种群分为亚群。然而，遗传距离与地理距离之间存在弱正相关。
