Herold C, Pflaum M, Utz P, Wilhelmi M, Rennekampff H-O, Vogt P M
Medizinische Hochschule Hannover, Klinik für Plastische, Hand- und Wiederherstellungschirurgie, Zentrum für Schwerbrandverletzte, Hannover.
Handchir Mikrochir Plast Chir. 2011 Dec;43(6):361-7. doi: 10.1055/s-0031-1284380. Epub 2012 Jan 12.
Various methods for harvesting and refining autologous fat grafts have been described. One of the standard procedures, the Coleman technique, is based on manual aspiration to reduce the negative presssure and the centrifugation of the grafts. The Shippert technique uses automatic liposuction with reduced negative pressure and abstains from centifugation in order not to reduce viability of the graft by exposing it to centrifugal forces. This study intends to compare the viability of fat grafts processed with the above-mentioned methods.Fat grafts were obtained in 9 patients by using both the Tissu Trans system (Shippert technique) and the Coleman technique. To evaluate the impact of centrifugation forces, the grafts harvested with the Coleman technique were treated with standard adjustment of the centrifuge and also with doubled g-force. Viability of fat grafts was analysed with the WST-8 test and with annexin V/PI assay FACS analysis.The viability of fat grafts processed by the Coleman technique was significantly higher compared to the Shippert technique on applying the WST-8 test. Applying the annexin V/PI analysis, the viability of fat grafts was almost equal with both techniques. Whereas the fat grafts processed with the Tissu Trans system are injected without condensation, the grafts refined with the Coleman technique were concentrated 3 times by centrifugation compared to the primary liposuctioned graft volumes.The Coleman technique allows the preparation of a fat graft containing more viable cells than the Shippert technique. This is in part due to the condensation of the graft by centrifugation using the Coleman technique. The factor of condensation of the grafts harvested and refined with the Coleman technique exceeds the factor of increased fat graft viability in comparison to the Shippert technique. The Tissu Trans system is more than twice as fast and easier to use with a preferential use for large volume grafts like in breast augmentation, whereas the Coleman technique produces a more condensed graft, favouring it for fat grafting to the face where less volume is needed.
已有多种获取和提纯自体脂肪移植物的方法被描述。标准程序之一的科尔曼技术，是基于手动抽吸以降低负压，并对移植物进行离心。希珀特技术采用负压降低的自动吸脂法，且不进行离心，以免因离心力作用而降低移植物的活力。本研究旨在比较用上述方法处理的脂肪移植物的活力。通过使用组织传输系统（希珀特技术）和科尔曼技术，从9名患者身上获取脂肪移植物。为评估离心力的影响，用科尔曼技术获取的移植物分别采用离心机的标准设置处理以及双倍重力处理。通过WST - 8测试和膜联蛋白V/碘化丙啶（PI）分析流式细胞术分析脂肪移植物的活力。在应用WST - 8测试时，与希珀特技术相比，用科尔曼技术处理的脂肪移植物的活力显著更高。应用膜联蛋白V/PI分析时，两种技术处理的脂肪移植物的活力几乎相同。用组织传输系统处理的脂肪移植物注射时无需浓缩，而用科尔曼技术提纯的移植物与初次吸脂的移植物体积相比，通过离心浓缩了3倍。科尔曼技术能够制备出比希珀特技术含有更多活细胞的脂肪移植物。这部分归因于使用科尔曼技术通过离心对移植物进行浓缩。与希珀特技术相比，用科尔曼技术获取和提纯的移植物的浓缩系数超过了脂肪移植物活力增加的系数。组织传输系统的速度快两倍多，且更易于使用，尤其适用于如隆胸等大量移植物的情况，而科尔曼技术能产生更浓缩的移植物，更适合用于面部脂肪移植，因为面部所需的移植物体积较小。
