Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Environ Sci Technol. 2012 Feb 7;46(3):1546-55. doi: 10.1021/es203356z. Epub 2012 Jan 26.
In this study, the effect of different operational conditions on biofilm development and nitrification in three moving-bed biofilm reactors (MBBRs) was investigated: two reactors were operated in a continuously fed regime and one in sequencing-batch mode. The presence of organic carbon reduced the time required to form stable nitrifying biofilms. Subsequent stepwise reduction of influent COD caused a decrease in total polysaccharide and protein content, which was accompanied by a fragmentation of the biofilm, as shown by scanning electron microscopy, and by an enrichment of the biofilm for nitrifiers, as observed by fluorescent in situ hybridization (FISH) analysis. Polysaccharide and protein concentrations proved to be good indicators of biomass development and detachment in MBBR systems. Ammonium- and nitrite-oxidizing bacteria activities were affected when a pulse feeding of 4 g of NH(4)-N/(m(2)·day) was applied. Free nitrous acid and free ammonia were likely the inhibitors for ammonium- and nitrite-oxidizing bacteria.
在这项研究中，考察了三种移动床生物膜反应器（MBBR）中不同操作条件对生物膜发展和硝化作用的影响：两个反应器采用连续进料方式运行，一个采用序批式运行。有机碳的存在减少了形成稳定硝化生物膜所需的时间。随后逐步降低进水 COD 导致总多糖和蛋白质含量降低，扫描电子显微镜观察到生物膜的碎片化，荧光原位杂交（FISH）分析观察到生物膜中硝化菌的富集。多糖和蛋白质浓度被证明是 MBBR 系统中生物量发展和脱落的良好指标。当施加 4 g NH4-N/（m2·天）脉冲进料时，氨氧化菌和亚硝酸盐氧化菌的活性受到影响。游离亚硝酸和游离氨可能是氨氧化菌和亚硝酸盐氧化菌的抑制剂。
