具有群体感应和抗鱼类病原菌生物膜活性的叶际细菌。
Phyllosphere bacteria with antiquorum sensing and antibiofilm activities against fish pathogenic bacteria.
机构信息
Department of Biotechnology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Jenderal Sudirman 51, Jakarta, 12930, Indonesia.
Department of Aquatic Resources Management, Faculty of Marine Science and Fisheries, University of Udayana, Denpasar, Bali, 80361, Indonesia.
出版信息
BMC Res Notes. 2024 Jan 2;17(1):5. doi: 10.1186/s13104-023-06657-9.
OBJECTIVE
This research aims to quantify antiquorum sensing and antibiofilm activity of f phyllosphere bacteria against biofilm formed by pathogenic fish bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi.
RESULTS
Antiquorum sensing assay using Chromobacter violaceum as indicator bacteria and antibiofilm assay showed six phyllosphere bacteria have antiquorum sensing and antibiofilm activities against tested bacteria. The highest inhibition and destruction activity was showed by metabolite of JB 3B and EJB 5 F against A. hydrophila, respectively. Determination using light microscope and scanning electron microscope performed decreaing in biomass of biofilm observed after treated with metabolite from phyllosphere bacteria.
目的
本研究旨在量化水生植物细菌的群体感应和抗生物膜活性，以对抗致病性鱼类细菌（如嗜水气单胞菌、无乳链球菌和哈维氏弧菌）形成的生物膜。
结果
使用类铜绿假单胞菌作为指示菌的群体感应测定和抗生物膜测定表明，六种生境细菌具有群体感应和抗生物膜活性，可对抗测试细菌。JB3B 和 EJB5F 的代谢物对嗜水气单胞菌的抑制和破坏活性最高。用生物显微镜和扫描电子显微镜观察，发现用生境细菌代谢物处理后，生物膜的生物量减少。
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