Olson J W, Russell D H
Cancer Res. 1979 Aug;39(8):3074-9.
After a single injection of diethylnitrosamine (200 mg/kg), there was a rapid increase in the activity ratio of hepatic cyclic adenosine 3':5'-monophosphate (cyclic AMP)-dependent protein kinase (within 1 hr) followed by the induction of ornithine decarboxylase which was detectable by 3 hr. Both the cyclic AMP-dependent protein kinase activity ratio and the activity of ornithine decarboxylase were significantly elevated above controls for 7 days following the administration of diethylnitrosamine. A single noncarcinogenic dose of diethylnitrosamine (25 mg/kg) did not increase the cyclic AMP-dependent protein kinase activity ratio or induce ornithine decarboxylase activity at 24 hr postadministration. However, serial administration of diethylnitrosamine (25 mg/kg) for 4 or 7 days resulted in an increased activity ratio of cyclic AMP-dependent protein kinase and increased ornithine decarboxylase activity. This is the first report of a prolonged increase in both the activity ratio of hepatic cyclic AMP-dependent protein kinase and the activity of ornithine decarboxylase in response to a single carcinogenic dose of diethylnitrosamine.
单次注射二乙基亚硝胺（200毫克/千克）后，肝环磷腺苷（cAMP）依赖性蛋白激酶的活性比值迅速升高（1小时内），随后鸟氨酸脱羧酶被诱导，3小时时可检测到。在给予二乙基亚硝胺后的7天内，cAMP依赖性蛋白激酶活性比值和鸟氨酸脱羧酶活性均显著高于对照组。单次非致癌剂量的二乙基亚硝胺（25毫克/千克）在给药后24小时并未增加cAMP依赖性蛋白激酶活性比值或诱导鸟氨酸脱羧酶活性。然而，连续4天或7天给予二乙基亚硝胺（25毫克/千克）会导致cAMP依赖性蛋白激酶活性比值增加以及鸟氨酸脱羧酶活性增加。这是首次报道单次致癌剂量的二乙基亚硝胺会使肝cAMP依赖性蛋白激酶活性比值和鸟氨酸脱羧酶活性长期增加。
