Laboratório de Pesquisa em Produtos Naturais, Universidade Regional do Cariri, Crato-CE, Brazil.
Fitoterapia. 2012 Apr;83(3):508-12. doi: 10.1016/j.fitote.2011.12.024. Epub 2012 Jan 8.
Lippia sidoides Cham. (Verbenaceae) is used in the folk medicine as topical antiseptic in skin and mucous membranes and its therapeutic effect is attributed to the thymol presence. The objective of this work was to verify the chemical composition and antibiotic modifying activity of the essential oil extracted from the leaves of L. sidoides and its major component thymol. The essential oil was obtained by hydrodistillation and analyzed by GC/MS. The synergistic activity was evaluated using gaseous contact method. The essential oil was obtained (yield of 1.06%) and the GC/MS analysis identified the main constituents: thymol (84.9%) and p-cymene (5.33%). The antibiotic modifying activity was verified using the minimal inhibitory dose method and gaseous contact. It verified the interference of essential oil and thymol against all tested aminoglycosides. There were no statistical differences between the activity of the essential oil and thymol against Pseudomonas aeruginosa, indicating this to be the responsible composition for such activity. However, the oil was shown more effective when compared to the thymol against Staphylococcus aureus. The essential oil of L. sidoides and its major component thymol influence the activity of aminoglycosides and may be used as adjuvant in antibiotic therapy against respiratory tract bacterial pathogens.
唇形科（Verbenaceae）植物甜罗勒（Lippia sidoides Cham.）被民间医学用作皮肤和粘膜的局部防腐剂，其治疗效果归因于百里香酚的存在。本工作的目的是验证从甜罗勒叶中提取的精油及其主要成分百里香酚的化学成分和抗生素修饰活性。通过水蒸馏法获得精油，并通过 GC/MS 进行分析。协同活性通过气态接触法进行评估。获得了精油（收率为 1.06%），并通过 GC/MS 分析确定了主要成分：百里香酚（84.9%）和对伞花烃（5.33%）。通过最小抑菌剂量法和气态接触法验证了抗生素修饰活性。结果表明，精油和百里香酚均能干扰所有测试的氨基糖苷类药物。精油和百里香酚对铜绿假单胞菌的活性无统计学差异，表明这是该活性的主要成分。然而，与百里香酚相比，该油对金黄色葡萄球菌的作用更有效。甜罗勒的精油及其主要成分百里香酚影响氨基糖苷类药物的活性，可作为抗生素治疗呼吸道细菌病原体的辅助剂。
