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基于负载百里香和牛至精油的浓缩胶原蛋白水解物的纳米纤维的生物活性特性

Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils.

作者信息

Berechet Mariana Daniela, Gaidau Carmen, Miletic Aleksandra, Pilic Branka, Râpă Maria, Stanca Maria, Ditu Lia-Mara, Constantinescu Rodica, Lazea-Stoyanova Andrada

机构信息

Division Leather and Footwear Research Institute, National Research and Development Institute for Textiles and Leather, 031215 Bucharest, Romania.

Faculty of Technology, University of Novi Sad, 21102 Novi Sad, Serbia.

出版信息

Materials (Basel). 2020 Apr 1;13(7):1618. doi: 10.3390/ma13071618.

DOI:10.3390/ma13071618
PMID:32244692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7178294/
Abstract

This research aimed to obtain biocompatible and antimicrobial nanofibres based on concentrated collagen hydrolysate loaded with thyme or oregano essential oils as a natural alternative to synthesis products. The essential oils were successfully incorporated using electrospinning process into collagen resulting nanofibres with diameter from 471 nm to 580 nm and porous structure. The presence of essential oils in collagen nanofibre mats was confirmed by Attenuated Total Reflectance -Fourier Transform Infrared Spectroscopy (ATR-FTIR), Ultraviolet-visible spectroscopy (UV-VIS) and antimicrobial activity. Scanning Electron Microscopy with Energy Dispersive Spectroscopy analyses allowed evaluating the morphology and constituent elements of the nanofibre networks. Microbiological tests performed against and showed that the presence of essential oils supplemented the new collagen nanofibres with antimicrobial properties. The biocompatibility of collagen and collagen with essential oils was assessed by in vitro cultivation with NCTC clone 929 of fibroblastic cells and cell viability measurement. The results showed that the collagen and thyme or oregano oil composites have no cytotoxicity up to concentrations of 1000 μg·mL and 500 μg mL, respectively. Optimization of electrospinning parameters has led to the obtaining of new collagen electrospun nanofibre mats loaded with essential oils with potential use for wound dressings, tissue engineering or protective clothing.

摘要

本研究旨在制备基于负载百里香或牛至精油的浓缩胶原蛋白水解物的生物相容性抗菌纳米纤维，作为合成产品的天然替代品。通过静电纺丝工艺成功地将精油掺入胶原蛋白中，得到直径为471纳米至580纳米且具有多孔结构的纳米纤维。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、紫外可见光谱（UV-VIS）和抗菌活性证实了胶原蛋白纳米纤维垫中精油的存在。带有能谱分析的扫描电子显微镜用于评估纳米纤维网络的形态和组成元素。针对金黄色葡萄球菌和大肠杆菌进行的微生物测试表明，精油的存在赋予了新型胶原蛋白纳米纤维抗菌性能。通过与成纤维细胞的NCTC克隆929进行体外培养并测量细胞活力，评估了胶原蛋白以及含精油胶原蛋白的生物相容性。结果表明，胶原蛋白与百里香油或牛至油的复合材料在浓度分别高达1000微克·毫升和500微克·毫升时均无细胞毒性。静电纺丝参数的优化已导致获得负载精油的新型胶原蛋白静电纺纳米纤维垫，其具有用于伤口敷料、组织工程或防护服的潜在用途。

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