Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, People's Republic of China.
FEBS Lett. 2012 Feb 17;586(4):403-10. doi: 10.1016/j.febslet.2011.12.038. Epub 2012 Jan 11.
In this study, we identified a variable region in the C-terminus of hemocyanin from the shrimp Litopenaeus vannamei (2288-2503bp, HcSC) by sequence alignments. A total of 13 SNPs were identified by PCR-SSCP and HcSC clone sequencing. The SSCP patterns of HcSC could be modulated in Vibro parahaemolyticus-treated shrimps. A novel SSCP band with four SNP sites was identified in V. parahaemolyticus-resistant shrimps. More importantly, three of these four SNPs introduced variations in amino acid sequence and possibly secondary structure of the HcSC polypeptide and resulted in a higher agglutinative activity against seven pathogenic bacteria. These results suggest that the C-terminus of shrimp L. vannamei hemocyanin possesses SNPs, which may be related to shrimp resistance to different pathogens.
在这项研究中，我们通过序列比对鉴定出南美白对虾血蓝蛋白 C 端的一个可变区（2288-2503bp，HcSC）。通过 PCR-SSCP 和 HcSC 克隆测序共鉴定出 13 个 SNP。SSCP 模式可在副溶血弧菌处理的虾中调节。在副溶血弧菌抗性虾中鉴定到具有四个 SNP 位点的新型 SSCP 带。更重要的是，这四个 SNP 中的三个导致了 HcSC 多肽的氨基酸序列和可能的二级结构发生变化，从而对七种致病菌表现出更高的凝集活性。这些结果表明，南美白对虾血蓝蛋白的 C 端具有 SNP，这可能与虾对不同病原体的抗性有关。
