Madison R, Macklis J D, Thies C
Department of Neurobiology, Duke University Medical Center, Durham, NC 27710.
Brain Res. 1990 Jul 2;522(1):90-8. doi: 10.1016/0006-8993(90)91581-z.
A wide range of latex particles are described which are capable of carrying high concentrations of fluorescent dyes, drugs, and photoactive agents selectively to subpopulations of neurons in vitro and in vivo. Particle size, charge, and concentration were all found to influence uptake into cultured neurons or retrograde transport in vivo. Chromophore loadings of greater than 14% (w/w) were obtained. Incorporation of a photoactivated dye (chlorin e6) into the polymer did not compromise the ability of the dye to produce singlet oxygen following light exposure. We refer to this unique family of latex particles as the latex nanosphere delivery system (LNDS). The LNDS will be usefull for studies of neuroanatomy and nervous system development, as well as more general areas of biomedical research where it is desirable to selectively label subpopulations of cells. The LNDS also offers a means of providing targeted delivery of drugs or photoactive agents to selected subpopulations of cells; this will allow experimentation not currently possible using any existent methodology.
本文描述了多种乳胶颗粒，它们能够在体外和体内将高浓度的荧光染料、药物和光活性剂选择性地运送到神经元亚群。研究发现，颗粒大小、电荷和浓度都会影响其被培养神经元摄取或在体内的逆行运输。发色团负载量超过了14%（重量/重量）。将光活化染料（二氢卟吩e6）掺入聚合物中，并不影响该染料在光照后产生单线态氧的能力。我们将这一独特的乳胶颗粒家族称为乳胶纳米球递送系统（LNDS）。LNDS将有助于神经解剖学和神经系统发育的研究，以及生物医学研究中更广泛的领域，即需要选择性标记细胞亚群的领域。LNDS还提供了一种将药物或光活性剂靶向递送至选定细胞亚群的方法；这将使目前使用任何现有方法都无法进行的实验成为可能。
