Katz L C, Iarovici D M
Laboratory of Neurobiology, Rockefeller University, New York, NY 10021.
Neuroscience. 1990;34(2):511-20. doi: 10.1016/0306-4522(90)90159-2.
The properties of a new fluorescent retrograde neuronal tracer, green fluorescent latex microspheres ("beads"), are described. Green beads, like the red beads that have been in use for several years, are readily transported by neurons in the mammalian central nervous system. After survival times ranging from 24 h to over one month they produce a distinct granular green fluorescence in the neuronal cytoplasm that is highly resistant to fading, when viewed using fluorescein filter sets. Green and red beads are transported with similar efficacy; cells labelled with either one or both tracers are easily distinguished. When injected into the brain, green beads, like red beads, show only minimal diffusion from the injection site. Because these two tracers differ only in their fluorescence, they should be ideal for double-labelling studies in a wide variety of systems and in developmental studies in which long-term retention of labels is required.
本文描述了一种新型荧光逆行神经元示踪剂——绿色荧光乳胶微球（“珠子”）的特性。绿色珠子与已使用数年的红色珠子一样，很容易被哺乳动物中枢神经系统中的神经元运输。在存活时间从24小时到一个多月不等后，当使用荧光素滤光片组观察时，它们会在神经元细胞质中产生明显的颗粒状绿色荧光，且这种荧光非常抗褪色。绿色和红色珠子的运输效率相似；用其中一种或两种示踪剂标记的细胞很容易区分。当注入大脑时，绿色珠子与红色珠子一样，仅从注射部位有极少的扩散。由于这两种示踪剂仅在荧光方面有所不同，它们应该是各种系统中双标记研究以及需要长期保留标记的发育研究的理想选择。
