Department of Metabolic Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan.
FEBS Lett. 2012 Feb 17;586(4):368-72. doi: 10.1016/j.febslet.2012.01.001. Epub 2012 Jan 10.
In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.
在这项研究中，我们旨在确定 G 蛋白偶联受体 84（GPR84）在脂肪组织中的生理作用，以及其特定配体中链脂肪酸（MCFAs）。在小鼠中，高脂肪饮食上调了脂肪垫中 GPR84 的表达。在 3T3-L1 脂肪细胞中，与巨噬细胞系 RAW264 共培养或 TNFα 显著增强了 GPR84 的表达。在 TNFα 存在的情况下，MCFAs 下调了 3T3-L1 脂肪细胞中脂联素 mRNA 的表达。总之，我们的研究结果表明，GPR84 在脂肪细胞中出现是对浸润巨噬细胞中 TNFα 的反应，并加剧了肥胖和糖尿病之间的恶性循环。
