海胆胚胎中存在一种类似于小鼠uvomorulin（E-钙黏蛋白）的细胞黏附蛋白的免疫学证据。

Immunological evidence for the presence in sea urchin embryos of a cell adhesion protein similar to mouse uvomorulin (E-cadherin).

作者信息

Ghersi G, Vittorelli M L

机构信息

Department of Cellular and Developmental Biology, Palermo, Italy.

出版信息

Cell Differ Dev. 1990 Jul;31(1):67-75. doi: 10.1016/0922-3371(90)90091-a.

DOI:10.1016/0922-3371(90)90091-a

PMID:2224574

Abstract

A tryptic fragment (88 kDa), obtained from external digestion of sea urchin embryos carried out in the presence of calcium, shows immunological cross-reactivity with polyclonal and monoclonal antibodies (DECMA-1) against mouse teratocarcinoma uvomorulin. Fab fragments obtained from anti-mouse terato-carcinoma uvomorulin mono- and polyclonal antibodies, and from polyclonal antibodies against the partially purified 88-kDa tryptic fragment, decompact early sea urchin embryos and block reaggregation of dissociated sea urchin blastula cells. These data indicate the presence of an uvomorulin-like protein in sea urchin embryos and suggest an important role for this protein in embryonic development.

摘要

在钙存在的情况下对海胆胚胎进行体外消化得到的一个胰蛋白酶片段（88 kDa），与针对小鼠畸胎瘤uvomorulin的多克隆和单克隆抗体（DECMA-1）表现出免疫交叉反应性。从抗小鼠畸胎瘤uvomorulin单克隆和多克隆抗体以及针对部分纯化的88 kDa胰蛋白酶片段的多克隆抗体获得的Fab片段，能使早期海胆胚胎解聚，并阻止解离的海胆囊胚细胞重新聚集。这些数据表明海胆胚胎中存在一种uvomorulin样蛋白，并提示该蛋白在胚胎发育中起重要作用。