Noll H, Matranga V, Cascino D, Vittorelli L
Proc Natl Acad Sci U S A. 1979 Jan;76(1):288-92. doi: 10.1073/pnas.76.1.288.
Blastula embryos of the sea urchin Paracentrotus lividus, when dissociated into single cells by exposure to Ca2+- and Mg2+-free sea water, reassociate spontaneously to form aggregates capable of development to the final larval form (pluteus). This aggregation is prevented by Fab fragments obtained by immunization with purified membranes from blastula embryos. The inhibition was reversed by soluble proteins extracted with butanol from purified membranes or from intact cells. These extracts also strongly stimulated the rate of reaggregation of dissociated cells in the absence of Fab fragments. Exposure of dissociated cells to 2.5% (vol/vol) butanol removed completely the protein(s) responsible for reaggregation of the cells without impairing their viability. Reaggregation and embryonic development were completely restored to the extracted cells by readdition of the proteins extracted from either membranes or cells. Extracted cells from Paracentrotus could be reconstituted with proteins from Arbacia.
将海胆Paracentrotus lividus的囊胚胚胎暴露于无Ca2+和Mg2+的海水中使其解离成单个细胞后，这些细胞会自发重新聚集形成能够发育至最终幼虫形态（长腕幼虫）的聚集体。用囊胚胚胎的纯化膜免疫获得的Fab片段可阻止这种聚集。用丁醇从纯化膜或完整细胞中提取的可溶性蛋白可逆转这种抑制作用。在没有Fab片段的情况下，这些提取物还强烈刺激了解离细胞的重新聚集速率。将解离细胞暴露于2.5%（体积/体积）的丁醇中可完全去除负责细胞重新聚集的蛋白质，而不损害其活力。通过重新添加从膜或细胞中提取的蛋白质，可使提取细胞的重新聚集和胚胎发育完全恢复。Paracentrotus的提取细胞可用Arbacia的蛋白质进行重构。
