University of Florida-Whitney Laboratory for Marine Bioscience, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA.
J Exp Biol. 2012 Feb 1;215(Pt 3):559-65. doi: 10.1242/jeb.059626.
Honeybees learn to associate an odor with sucrose reward under conditions that allow the monitoring of neural activity by imaging Ca(2+) transients in morphologically identified neurons. Here we report such recordings from mushroom body extrinsic neurons - which belong to a recurrent tract connecting the output of the mushroom body with its input, potentially providing inhibitory feedback - and other extrinsic neurons. The neurons' responses to the learned odor and two novel control odors were measured 24 h after learning. We found that calcium responses to the learned odor and an odor that was strongly generalized with it were enhanced compared with responses to a weakly generalized control. Thus, the physiological responses measured in these extrinsic neurons accurately reflect what is observed in behavior. We conclude that the recorded recurrent neurons feed information back to the mushroom body about the features of learned odor stimuli. Other extrinsic neurons may signal information about learned odors to different brain regions.
在允许通过成像形态学鉴定神经元中的钙瞬变来监测神经活动的条件下，蜜蜂学会将一种气味与蔗糖奖励联系起来。在这里，我们报告了从蘑菇体外在神经元中进行的此类记录 - 这些神经元属于与蘑菇体的输出与其输入相连接的递归途径的一部分，可能提供抑制性反馈 - 以及其他外在神经元的记录。在学习后 24 小时测量了神经元对学习气味和两种新的对照气味的反应。我们发现，与对弱广义对照的反应相比，对学习气味和与其强烈广义的气味的钙反应得到了增强。因此，在这些外在神经元中测量的生理反应准确地反映了在行为中观察到的反应。我们得出结论，记录的递归神经元将与学习气味刺激相关的特征反馈给蘑菇体。其他外在神经元可能将关于学习气味的信息传递给不同的大脑区域。
