Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, PR China.
Oncol Rep. 2012 Apr;27(4):1238-44. doi: 10.3892/or.2012.1625. Epub 2012 Jan 11.
Dysregulation of microRNAs (miRNAs) has been found to be associated with a variety of diseases, including epithelial ovarian cancer (EOC). Recently, miR-100 was reported to be downregulated in human ovarian carcinoma, however, the clinical significance and functional roles of miR-100 expression in human EOC are unclear. TaqMan real-time quantitative RT-PCR assay was performed to detect the expression of miR-100 in 98 EOC tissues and 15 adjacent normal epithelial tissues. The relationship between miR-100 expression and clinicopathological factors in 98 EOC patients was statistically analyzed. The effect of miR-100 expression on patient survival was determined. Finally, the role of miR-100 in EOC cell growth and its possible mechanisms were analyzed with miR-100 precursor or inhibitor-transfected cells. We showed that the level of miR-100 was significantly lower in EOC tissues compared to adjacent normal tissues. Low miR-100 expression was found to be closely correlated with advanced FIGO stage, higher serum CA125 expression level and lymph node involvement. Also, low miR-100 expression was correlated with shorter overall survival of EOC patients, and multivariate analysis showed that the status of miR-100 expression was an independent predictor of overall survival in EOC. Additionally, miR-100 could affect the growth of EOC cells by post-transcriptionally regulating polo-like kinase 1 (PLK1) expression. Together, these results suggest that low miR-100 expression may be an independent poor prognostic factor and miR-100 can function as a tumor suppressor by targeting PLK1 in human EOCs.
miRNAs（微小 RNA）的失调已被发现与多种疾病相关，包括上皮性卵巢癌（EOC）。最近，有报道称 miR-100 在人卵巢癌中下调，然而，miR-100 表达在人 EOC 中的临床意义和功能作用尚不清楚。采用 TaqMan 实时定量 RT-PCR 法检测 98 例 EOC 组织和 15 例相邻正常上皮组织中 miR-100 的表达。统计分析 98 例 EOC 患者 miR-100 表达与临床病理因素的关系。确定 miR-100 表达对患者生存的影响。最后，通过转染 miR-100 前体或抑制剂的细胞分析 miR-100 在 EOC 细胞生长中的作用及其可能的机制。我们表明，与相邻正常组织相比，EOC 组织中 miR-100 的水平明显降低。发现低 miR-100 表达与 FIGO 晚期、血清 CA125 表达水平升高和淋巴结受累密切相关。此外，低 miR-100 表达与 EOC 患者总生存期较短相关，多变量分析表明 miR-100 表达状态是 EOC 患者总生存期的独立预后因素。此外，miR-100 可以通过对 polo-like kinase 1（PLK1）表达的转录后调节来影响 EOC 细胞的生长。总之，这些结果表明低 miR-100 表达可能是一个独立的不良预后因素，miR-100 可以通过靶向 PLK1 在人 EOC 中发挥肿瘤抑制作用。
