Liu Wei, Li Daihong, Liu Chun
Department of Blood Transfusion, First Central Hospital of Tianjin, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Dec;36(12):1213-6. doi: 10.3969/j.issn.1672-7347.2011.12.015.
Serologic characters of Bx subtype were analyzed with standard Methods , PCR-SSP method was used for its genotype, and DNA sequences of 6 and 7 exons of ABO genes were analyzed by DNA sequencing. Serologically, the anti-B test was no or 1+, with anti-AB (1+ or 2+). The patient's auto antibodies were negative. PCR-SSP showed the patient was Bx02/O2. ABO genetic locus sequencing was 261delG in exon 6 and 297 was GG homozygous. The sample was a combination of blood group B and O2 because 297A> G, 526C> G, 657C> T, 703G> A, 796C> A, 803G> C, 905A> G, 930A> G shown by direct DNA sequencing and 803G> C, 905A> G shown by cloning DNA sequencing. The serological phenotype of the specimen was B and the genetic sequence was a combination of ABOBx.02.1.1 and ABOO21.01.1.1.
采用标准方法分析Bx亚型的血清学特征，用聚合酶链反应-序列特异性引物（PCR-SSP）方法检测其基因型，通过DNA测序分析ABO基因第6和第7外显子的DNA序列。血清学检测显示，抗B试验结果为无反应或1+，抗AB为1+或2+。患者自身抗体阴性。PCR-SSP检测显示患者为Bx02/O2。ABO基因座测序结果显示第6外显子存在261delG，第297位为GG纯合子。直接DNA测序显示297A>G、526C>G、657C>T、703G>A、796C>A、803G>C、905A>G、930A>G，克隆DNA测序显示803G>C、905A>G，该样本为B血型和O2血型的组合。标本的血清学表型为B型，基因序列为ABOBx.02.1.1和ABOO21.01.1.1的组合。
