[三例罕见Bx02血型病例的血清学和分子研究]
[Serological and molecular study of three cases with a rare Bx02 blood group].
作者信息
Han Bin, Liu Peiyan, Liu Xiaohua, Feng Zhihui
机构信息
Institute of Transfusion Medicine, Qingdao Blood Center, Qingdao, Shandong 266071, China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2017 Feb 10;34(1):65-67. doi: 10.3760/cma.j.issn.1003-9406.2017.01.015.
OBJECTIVE
To determine the genotypes of three blood samples suspected as B subtype through DNA sequencing.
METHODS
The samples were first genotyped with PCR-SSP. Exons 6 and 7 of the ABO genes were subjected to PCR, direct sequencing, and cloned sequencing to determine the genotypes.
RESULTS
Serological results of the three samples were similar, with red cells being weakly agglutinatable by anti-B and serum containing anti-B. The samples were preliminarily genotyped as B/O1. Sequencing analysis showed that all three samples contained an O allele and a 905A>G mutation of the B gene, which was previously defined as Bx02.
CONCLUSION
Through sequencing analysis, the three samples typed as B subtype with serological testing were identified as Bx phenotype. The genotype of samples 1 and 2 was Bx02/O101, and that of sample 3 was Bx02/O102.
目的
通过DNA测序确定三份疑似B亚型的血样的基因型。
方法
首先用PCR-SSP对样本进行基因分型。对ABO基因的第6和第7外显子进行PCR、直接测序和克隆测序以确定基因型。
结果
三份样本的血清学结果相似，红细胞被抗B血清和含抗B的血清弱凝集。样本初步基因分型为B/O1。测序分析表明，所有三份样本均含有一个O等位基因和B基因的905A>G突变，该突变先前被定义为Bx02。
结论
通过测序分析，血清学检测为B亚型的三份样本被鉴定为Bx表型。样本1和2的基因型为Bx02/O101，样本3的基因型为Bx02/O102。
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