Li Ting, Si Zhongzhou, Qi Haizhi, He Zhijun, Li Yining
Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Dec;36(12):1147-52. doi: 10.3969/j.issn.1672-7347.2011.12.004.
To investigate the expression of T helper (Th) 17 cells and the related interleukin 17 (IL-17) in acute renal allograft rejection in mice and its significance.
We established a mouse renal allograft model, in which mice were randomly divided into a renal isograft group and an acute renal allograft rejection group. Three and 7 d after the transplantation, the serum interferon (IFN)-γ and IL-17 levels in the mice were determined by enzyme-linked immunosorbent assay, the percentage of Th1 and Th17 cells in the total kidney-infiltrating lymphocytes was investigated by flow cytometry, and the transplanted kidney species were given routine pathological examination after fixation with 10% formalin.
Compared with the isograft group, the allograft mice showed a significantly higher content of IL-17 (P<0.05) but not IFN-γ in the serum 3 d after transplantation, and showed significantly higher serum IL-17 and IFN-γ contents 7 d after transplantation (P<0.05). Also, compared with the isograft group, the allograft mice exhibited significantly higher percentage of Th1 and Th17 cells on both day 3 and day 7 (P<0.05). In the allograft group, the contents of serum IFN-γ and IL-17 and the percentage of Th1 and Th17 cells were significantly higher on day 7 than on day 3 (P<0.05). Routine pathological examination indicated that, as time passed, the allograft mice showed gradually stronger rejection responses.
Th17 cells might play an important role in the development of acute renal allograft rejection, and IL-17 can be used as an early indicator of acute rejection.
探讨辅助性T细胞（Th）17细胞及其相关白细胞介素17（IL-17）在小鼠急性肾移植排斥反应中的表达及其意义。
建立小鼠肾移植模型，将小鼠随机分为肾同基因移植组和急性肾移植排斥组。移植后3天和7天，采用酶联免疫吸附测定法测定小鼠血清干扰素（IFN）-γ和IL-17水平，采用流式细胞术检测移植肾中Th1和Th17细胞在总浸润淋巴细胞中的百分比，并用10%福尔马林固定后对移植肾进行常规病理检查。
与同基因移植组相比，移植后3天，同种异体移植小鼠血清中IL-17含量显著升高（P<0.05），而IFN-γ含量无明显变化；移植后7天，血清IL-17和IFN-γ含量均显著升高（P<0.05）。此外，与同基因移植组相比，同种异体移植小鼠在第3天和第7天Th1和Th17细胞的百分比均显著升高（P<0.05）。在同种异体移植组中，第7天血清IFN-γ和IL-17含量以及Th1和Th17细胞百分比均显著高于第3天（P<0.05）。常规病理检查表明，随着时间的推移，同种异体移植小鼠的排斥反应逐渐增强。
Th17细胞可能在急性肾移植排斥反应的发生中起重要作用，IL-17可作为急性排斥反应的早期指标。
