• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Role of CTCF in EGF-induced migration of immortalized human corneal epithelial cells.CTCF 在表皮生长因子诱导的永生化人角膜上皮细胞迁移中的作用。
Invest Ophthalmol Vis Sci. 2012 Feb 23;53(2):946-51. doi: 10.1167/iovs.11-8747. Print 2012 Feb.
2
Epidermal growth factor (EGF)-induced corneal epithelial wound healing through nuclear factor κB subtype-regulated CCCTC binding factor (CTCF) activation.表皮生长因子（EGF）通过核因子κB 亚基调控的 CCCTC 结合因子（CTCF）激活诱导角膜上皮伤口愈合。
J Biol Chem. 2013 Aug 23;288(34):24363-71. doi: 10.1074/jbc.M113.458141. Epub 2013 Jul 10.
3
CTCF-Mediated and Pax6-Associated Gene Expression in Corneal Epithelial Cell-Specific Differentiation.CTCF介导及Pax6相关基因表达在角膜上皮细胞特异性分化中的作用
PLoS One. 2016 Sep 1;11(9):e0162071. doi: 10.1371/journal.pone.0162071. eCollection 2016.
4
Epidermal growth factor-induced proliferation requires down-regulation of Pax6 in corneal epithelial cells.表皮生长因子诱导的增殖需要角膜上皮细胞中Pax6的下调。
J Biol Chem. 2005 Apr 1;280(13):12988-95. doi: 10.1074/jbc.M412458200. Epub 2005 Jan 19.
5
Reactive oxygen species (ROS) are essential mediators in epidermal growth factor (EGF)-stimulated corneal epithelial cell proliferation, adhesion, migration, and wound healing.活性氧（ROS）是表皮生长因子（EGF）刺激角膜上皮细胞增殖、黏附、迁移和伤口愈合过程中的重要介质。
Exp Eye Res. 2009 Dec;89(6):876-86. doi: 10.1016/j.exer.2009.07.012. Epub 2009 Jul 25.
6
Effect of EGF-induced HDAC6 activation on corneal epithelial wound healing.表皮生长因子诱导的组蛋白去乙酰化酶 6 激活对角膜上皮伤口愈合的影响。
Invest Ophthalmol Vis Sci. 2010 Jun;51(6):2943-8. doi: 10.1167/iovs.09-4639. Epub 2010 Jan 20.
7
Effect of CTCF-binding motif on regulation of PAX6 transcription.CTCF结合基序对PAX6转录调控的影响。
Invest Ophthalmol Vis Sci. 2006 Jun;47(6):2422-9. doi: 10.1167/iovs.05-0536.
8
Activation of oxidative stress-regulated Bcl-3 suppresses CTCF in corneal epithelial cells.氧化应激调节的 Bcl-3 激活抑制角膜上皮细胞中的 CTCF。
PLoS One. 2011;6(8):e23984. doi: 10.1371/journal.pone.0023984. Epub 2011 Aug 30.
9
Role of small GTPase Rho in regulating corneal epithelial wound healing.小GTP酶Rho在调节角膜上皮伤口愈合中的作用。
Invest Ophthalmol Vis Sci. 2008 Mar;49(3):900-9. doi: 10.1167/iovs.07-1122.
10
Lysophosphatidic acid promoting corneal epithelial wound healing by transactivation of epidermal growth factor receptor.溶血磷脂酸通过表皮生长因子受体的反式激活促进角膜上皮伤口愈合。
Invest Ophthalmol Vis Sci. 2007 Feb;48(2):636-43. doi: 10.1167/iovs.06-0203.

引用本文的文献

1
Corneal epithelial development and homeostasis.角膜上皮的发育和稳态。
Differentiation. 2023 Jul-Aug;132:4-14. doi: 10.1016/j.diff.2023.02.002. Epub 2023 Mar 1.
2
High-resolution promoter map of human limbal epithelial cells cultured with keratinocyte growth factor and rho kinase inhibitor.高分辨率人角膜缘上皮细胞启动子图谱的培养与角质细胞生长因子和 rho 激酶抑制剂。
Sci Rep. 2017 Jun 6;7(1):2845. doi: 10.1038/s41598-017-02824-8.
3
CTCF-Mediated and Pax6-Associated Gene Expression in Corneal Epithelial Cell-Specific Differentiation.CTCF介导及Pax6相关基因表达在角膜上皮细胞特异性分化中的作用
PLoS One. 2016 Sep 1;11(9):e0162071. doi: 10.1371/journal.pone.0162071. eCollection 2016.
4
Progress in corneal wound healing.角膜伤口愈合的进展。
Prog Retin Eye Res. 2015 Nov;49:17-45. doi: 10.1016/j.preteyeres.2015.07.002. Epub 2015 Jul 18.
5
Epidermal growth factor (EGF)-induced corneal epithelial wound healing through nuclear factor κB subtype-regulated CCCTC binding factor (CTCF) activation.表皮生长因子（EGF）通过核因子κB 亚基调控的 CCCTC 结合因子（CTCF）激活诱导角膜上皮伤口愈合。
J Biol Chem. 2013 Aug 23;288(34):24363-71. doi: 10.1074/jbc.M113.458141. Epub 2013 Jul 10.
6
Ocular surface development and gene expression.眼表发育与基因表达。
J Ophthalmol. 2013;2013:103947. doi: 10.1155/2013/103947. Epub 2013 Feb 21.

本文引用的文献

1
Activation of oxidative stress-regulated Bcl-3 suppresses CTCF in corneal epithelial cells.氧化应激调节的 Bcl-3 激活抑制角膜上皮细胞中的 CTCF。
PLoS One. 2011;6(8):e23984. doi: 10.1371/journal.pone.0023984. Epub 2011 Aug 30.
2
Regulation of Pax6 by CTCF during induction of mouse ES cell differentiation.CTCF 在诱导小鼠胚胎干细胞分化过程中对 Pax6 的调控。
PLoS One. 2011;6(6):e20954. doi: 10.1371/journal.pone.0020954. Epub 2011 Jun 13.
3
Hyperosmotic stress-induced corneal epithelial cell death through activation of Polo-like kinase 3 and c-Jun.高渗应激通过激活 Polo 样激酶 3 和 c-Jun 诱导角膜上皮细胞死亡。
Invest Ophthalmol Vis Sci. 2011 May 16;52(6):3200-6. doi: 10.1167/iovs.10-6485.
4
Gene expression analysis in SV-40 immortalized human corneal epithelial cells cultured with an air-liquid interface.在气液界面培养的SV - 40永生化人角膜上皮细胞中的基因表达分析
Mol Vis. 2010 Oct 15;16:2109-20.
5
Control of actin filament treadmilling in cell motility.细胞运动中肌动蛋白丝的 treadmilling 控制。
Annu Rev Biophys. 2010;39:449-70. doi: 10.1146/annurev-biophys-051309-103849.
6
NF-kappaB subtypes regulate CCCTC binding factor affecting corneal epithelial cell fate.NF-κB 亚型调节 CCCTC 结合因子影响角膜上皮细胞命运。
J Biol Chem. 2010 Mar 26;285(13):9373-9382. doi: 10.1074/jbc.M109.094425. Epub 2010 Jan 28.
7
Effect of EGF-induced HDAC6 activation on corneal epithelial wound healing.表皮生长因子诱导的组蛋白去乙酰化酶 6 激活对角膜上皮伤口愈合的影响。
Invest Ophthalmol Vis Sci. 2010 Jun;51(6):2943-8. doi: 10.1167/iovs.09-4639. Epub 2010 Jan 20.
8
ERK1/2 mediate wounding- and G-protein-coupled receptor ligands-induced EGFR activation via regulating ADAM17 and HB-EGF shedding.细胞外信号调节激酶1/2通过调控金属蛋白酶17（ADAM17）和肝素结合表皮生长因子（HB-EGF）的脱落来介导伤口和G蛋白偶联受体配体诱导的表皮生长因子受体（EGFR）激活。
Invest Ophthalmol Vis Sci. 2009 Jan;50(1):132-9. doi: 10.1167/iovs.08-2246. Epub 2008 Jul 24.
9
Functional role of CCCTC binding factor (CTCF) in stress-induced apoptosis.CCCTC结合因子（CTCF）在应激诱导的细胞凋亡中的功能作用。
Exp Cell Res. 2007 Aug 15;313(14):3057-65. doi: 10.1016/j.yexcr.2007.05.018. Epub 2007 May 24.
10
Role of c-Fos/JunD in protecting stress-induced cell death.c-Fos/JunD在保护应激诱导的细胞死亡中的作用。
Cell Prolif. 2007 Jun;40(3):431-44. doi: 10.1111/j.1365-2184.2007.00444.x.

CTCF 在表皮生长因子诱导的永生化人角膜上皮细胞迁移中的作用。

Role of CTCF in EGF-induced migration of immortalized human corneal epithelial cells.

机构信息

Department of Medicine, Jules Stein Eye Institute, David Geffen School of Medicine, University of California Los Angeles, CA 90502, USA.

出版信息

Invest Ophthalmol Vis Sci. 2012 Feb 23;53(2):946-51. doi: 10.1167/iovs.11-8747. Print 2012 Feb.

DOI:10.1167/iovs.11-8747
PMID:22247490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3317431/
Abstract

PURPOSE

EGF-induced activation of the epigenetic CCCTC binding factor (CTCF) plays an important role in corneal epithelial cell proliferation by suppressing the Pax6 gene. The present study focused on further understanding the role of CTCF in mediating EGF-induced migration of immortalized human corneal epithelial cells.

METHODS

CTCF activities in human corneal epithelial cells immortalized by telomerase (HTCE cells) and SV-40 (HCE cells) transformation were suppressed and enhanced by CTCF mRNA knockdown and by overexpressing CTCF cDNA, respectively. EGF-induced cell migration was evaluated by linear scratch wound healing, a cell migration assay, and live cell motility GFP-tracking with a fluorescence microscope. Immunochemical analysis was performed for detecting focal adhesion changes in EGF-induced and CTCF activity-altered cells.

RESULTS

EGF-induced wound closure and cell migration rates of human corneal epithelial cells were significantly suppressed and enhanced by CTCF mRNA knockdown and by overexpression of CTCF, respectively. CTCF mRNA knockdown also markedly suppressed cell motility, determined by using a live-cell-tracking system in GFP-tag-expressed HTCE cells. Finally, alterations of EGF-stimulated focal adhesion were observed in CTCF knockdown HTCE cells by immunostaining of F-actin and vinculin in cytoskeleton reorganization.

CONCLUSIONS

CTCF, an epigenetic regulator and transcription factor, involves EGF-induced increases in cell motility and migration. CTCF plays an essential role in growth factor-regulated human corneal epithelial cell wound healing.

摘要

目的

表皮生长因子（EGF）诱导的表观遗传 CCCTC 结合因子（CTCF）的激活通过抑制 Pax6 基因在角膜上皮细胞增殖中发挥重要作用。本研究旨在进一步了解 CTCF 在介导永生化人角膜上皮细胞中 EGF 诱导的迁移中的作用。

方法

通过 CTCF mRNA 敲低和过表达 CTCF cDNA 分别抑制和增强端粒酶（HTCE 细胞）和 SV-40（HCE 细胞）转化的人角膜上皮细胞中的 CTCF 活性。通过线性划痕愈合、细胞迁移测定和荧光显微镜下 GFP 标记的活细胞运动追踪评估 EGF 诱导的细胞迁移。进行免疫化学分析以检测 EGF 诱导和 CTCF 活性改变细胞中的焦点粘连变化。

结果

EGF 诱导的人角膜上皮细胞的伤口闭合和细胞迁移率分别通过 CTCF mRNA 敲低和 CTCF 的过表达显著抑制和增强。CTCF mRNA 敲低也显著抑制了 GFP 标记的 HTCE 细胞中活细胞追踪系统测定的细胞运动。最后，通过细胞骨架重排中的 F-肌动蛋白和纽蛋白的免疫染色观察到 CTCF 敲低 HTCE 细胞中 EGF 刺激的焦点粘连的改变。

结论

作为一种表观遗传调节剂和转录因子，CTCF 参与 EGF 诱导的细胞迁移和迁移增加。CTCF 在生长因子调节的人角膜上皮细胞伤口愈合中起重要作用。