Baumgart J, Gase K, Schulze W, Suehnel J, Gutsche W, Behnke D
Academy of Sciences, Central Institute of Microbiology and Experimental Therapy, Jena, Germany.
Cancer Lett. 1990 Nov 5;54(3):119-24. doi: 10.1016/0304-3835(90)90032-s.
Dihydroambazone (DHA) is a water-soluble derivative of the experimental anticancer drug ambazone. In vitro, a combination of DHA and human recombinant tumor necrosis factor alpha (TNF) exerted a strong synergism of cytotoxicity against both mouse melanoma B16K cells and the TNF-sensitive mouse fibroblast line L-M (S). Furthermore, in a colony-forming assay with B16K cells a combination of TNF and DHA inhibited colony-formation much more severely than either drug alone. An increased antiproliferative efficiency was also confirmed in vivo against established subcutaneous melanoma B16 tumors of C57BL/6 mice.
二氢安巴腙（DHA）是实验性抗癌药物安巴腙的水溶性衍生物。在体外，DHA与重组人肿瘤坏死因子α（TNF）联合使用对小鼠黑色素瘤B16K细胞和TNF敏感的小鼠成纤维细胞系L-M（S）均表现出强烈的细胞毒性协同作用。此外，在B16K细胞的集落形成试验中，TNF和DHA联合使用比单独使用任何一种药物更严重地抑制了集落形成。在体内对C57BL/6小鼠已建立的皮下黑色素瘤B16肿瘤的抗增殖效率也得到了提高。
