Chang Dennis, Florea Andrew, Rowe Mark, Seiberling Kristin A
Department of Otolaryngology-Head and Neck Surgery, Loma Linda University, 11234 Anderson St., Loma Linda, CA 92354, USA.
Arch Otolaryngol Head Neck Surg. 2012 Feb;138(2):119-21. doi: 10.1001/archoto.2011.1204. Epub 2012 Jan 16.
To determine the efficacy of various cleaning and disinfective methods in reducing bacterial and fungal load on flexible fiberoptic laryngoscopes (FFLs).
In vitro model.
Flexible fiberoptic laryngoscopes contaminated with Staphylococcus aureus and Candida albicans.
Contamination with S aureus and C albicans was separately induced on FFLs, which were then disinfected with different protocols: 20-, 15-, 10-, and 5-minute soaks in ortho-phthalaldehyde (Cidex OPA; Johnson & Johnson) with or without presoaking in an enzymatic soap solution for 5 minutes; an isolated 5-minute soak in an enzymatic soap solution; a 30-second wipe with antibacterial soap and water; a 30-second wipe with isopropyl alcohol; a 30-second wipe with antibacterial soap, followed by a 30-second scrub with isopropyl alcohol; and a 30-second wipe with germicidal cloth, all accompanied by previous rinsing with 30 seconds of running tap water.
All protocols except the isolated 5-minute soak in enzymatic soap solution were successful in completely disinfecting the FFLs after experimental contamination with S aureus or C albicans.
Various different cleaning methods appeared to properly disinfect FFLs after inoculation with S aureus and C albicans in an in vitro model.
确定各种清洁和消毒方法在降低软性纤维喉镜（FFL）上细菌和真菌负荷方面的效果。
体外模型。
被金黄色葡萄球菌和白色念珠菌污染的软性纤维喉镜。
在FFL上分别诱导金黄色葡萄球菌和白色念珠菌污染，然后用不同方案进行消毒：在邻苯二甲醛（Cidex OPA；强生公司）中浸泡20分钟、15分钟、10分钟和5分钟，浸泡前有无在酶皂溶液中预浸泡5分钟；单独在酶皂溶液中浸泡5分钟；用抗菌皂和水擦拭30秒；用异丙醇擦拭30秒；先用抗菌皂擦拭30秒，然后用异丙醇擦洗30秒；用杀菌布擦拭30秒，所有操作前均先用流动自来水冲洗30秒。
除单独在酶皂溶液中浸泡5分钟外，所有方案在金黄色葡萄球菌或白色念珠菌实验污染后均成功完全消毒FFL。
在体外模型中，各种不同的清洁方法在接种金黄色葡萄球菌和白色念珠菌后似乎能正确消毒FFL。
