Department of Drug Sciences, University of Pavia, Pavia 27100, Italy.
Immunohaematology and Transfusion Service, Apheresis and Cell Therapy Unit, IRRCS Policlinico San Matteo, Pavia 27100, Italy.
Int J Pharm. 2012 Apr 15;426(1-2):1-6. doi: 10.1016/j.ijpharm.2011.12.059. Epub 2012 Jan 10.
Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact of platelet lysate (PL) with cornea ulcers. A sterile vehicle based on chondroitin sulphate sodium (CS) and hydroxypropylmethyl cellulose (HPMC) was developed. An extemporaneous loading of the vehicle with PL was performed and the obtained formulation was able to quickly thermogelify at about 32 °C and was characterized by good mucoadhesive properties. ELISA evidenced that the growth factor PDGF AB was compatible with the vehicle and stable in the formulation up to 15 days of storage at 2-8 °C. In vitro wound healing and proliferation test (performed using rabbit corneal epithelial cells (RCE)) showed that the formulation enhanced cell growth and put in evidence a synergistic effect of CS and PL in stimulating cell proliferation. The overall results indicate that PL loaded in thermosensitive and mucoadhesive eyedrops can be profitably employed to treat corneal lesions.
角膜病变会导致严重的疼痛和视力障碍，在许多情况下，对常规治疗反应不佳。血小板裂解液（PL）是一种富含生长因子（GFs）的血液衍生物，这些生长因子是在富含血小板的血浆（PRP）经冻融破坏后由血小板释放出来的。本研究的目的是开发热敏和黏膜粘附性滴眼剂，以维持和延长血小板裂解液（PL）与角膜溃疡的接触。开发了一种基于硫酸软骨素钠（CS）和羟丙基甲基纤维素（HPMC）的无菌载体。对载体进行了 PL 的临时加载，所得制剂能够在约 32°C 快速形成温敏凝胶，并且具有良好的黏膜粘附性能。ELISA 表明生长因子 PDGF AB 与载体相容，在 2-8°C 下储存 15 天内稳定。体外伤口愈合和增殖试验（使用兔角膜上皮细胞（RCE）进行）表明，该制剂促进了细胞生长，并显示出 CS 和 PL 在刺激细胞增殖方面具有协同作用。总体结果表明，负载在热敏和黏膜粘附性滴眼剂中的 PL 可有效地用于治疗角膜病变。
