Desmoulins D, Ponteziere C, Agneray J, Ekindjian O G, Cals M J
Laboratoire de Biochimie, CNRS UA DO 622, Universite Paris XI, Chatenay-Malbary, France.
Cell Mol Biol. 1990;36(3):309-16.
The growth of adherent synovial cells passaged once was studied in response to human recombinant interleukin 1 (hr IL-1) beta. Human synovial cell cultures were established from tissues obtained during therapeutic joint surgery for patients with rheumatoid arthritis (rheumatoid synovial cells, RSC) or non inflammatory rheumatic diseases (non rheumatoid synovial cells, NRSC). The effect of IL-1 beta (0.1 to 10 ng/ml) on the time course of proliferation showed that values for DNA synthesis and cell numbers in RSC cultures were higher than in NRSC cultures. Similarly, untreated control RSC cultures grew more quickly than NRSC. These results demonstrate that RSC, which are continuously stimulated by IL-1 beta produced in the rheumatoid pannus in vivo, have a higher capacity for proliferation than NRSC but are less responsive to IL-1 beta. A dose-response curve of proliferation was established 72 hrs. after the addition of IL-1 to the medium. The stimulating effect of IL-1 beta (0.001 to 10 ng/ml) was dose-dependent in both RSC and NRSC and reached a plateau at 10 ng/ml; the response of NRSC was stronger than that of RCS.
研究了传代一次的贴壁滑膜细胞对人重组白细胞介素1（hr IL-1）β的反应。从类风湿性关节炎患者（类风湿滑膜细胞，RSC）或非炎性风湿性疾病患者（非类风湿滑膜细胞，NRSC）治疗性关节手术中获取的组织建立人滑膜细胞培养物。IL-1β（0.1至10 ng/ml）对增殖时间进程的影响表明，RSC培养物中DNA合成和细胞数量的值高于NRSC培养物。同样，未处理的对照RSC培养物比NRSC生长得更快。这些结果表明，在体内被类风湿血管翳中产生的IL-1β持续刺激的RSC，比NRSC具有更高的增殖能力，但对IL-1β的反应性较低。在向培养基中添加IL-1后72小时建立增殖的剂量反应曲线。IL-1β（0.001至10 ng/ml）的刺激作用在RSC和NRSC中均呈剂量依赖性，并在10 ng/ml时达到平台期；NRSC的反应比RCS更强。
