Hernvann A, Cynober L, Aussel C, Ekindjian O G
Laboratoire de Biochimie, Université Paris XI, Chatenay-Malabry, France.
Cell Mol Biol. 1991;37(5):541-7.
Basal and insulin-mediated glucose uptake were studied in human synovial cells. Cell cultures were established from samples of synovium obtained at synovectomy from patients with osteoarthritis (non-rheumatoid synovial cells, NRSC) or rheumatoid arthritis (rheumatoid synovial cells, RSC). Basal glucose uptake was significantly higher in RSC than in NRSC. NRSC were sensitive to insulin at near-physiological concentrations (10(-8)M), with maximal transport occurring after a 30-min. association time (27.2 +/- 2.0%, mean +/- SEM). Insulin did not stimulate significantly 2-déoxy-D-glucose uptake in RSC, regardless of the association time (up to 120 min.) or the insulin concentration (10(-10) to 10(-6) M). Treatment of NRSC with human recombinant interleukin-1 beta (IL-1 beta) enhanced glucose uptake to a level similar to basal uptake by RSC. These results suggest that autocrine production of IL-1 beta by RSC could be responsible for the higher basal glucose uptake by these cells.
在人滑膜细胞中研究了基础葡萄糖摄取和胰岛素介导的葡萄糖摄取。从骨关节炎患者（非类风湿性滑膜细胞，NRSC）或类风湿性关节炎患者（类风湿性滑膜细胞，RSC）滑膜切除术中获取的滑膜样本建立细胞培养物。RSC中的基础葡萄糖摄取显著高于NRSC。NRSC对接近生理浓度（10^(-8)M）的胰岛素敏感，在30分钟的结合时间后出现最大转运（27.2±2.0%，平均值±标准误）。无论结合时间（长达120分钟）或胰岛素浓度（10^(-10)至10^(-6)M）如何，胰岛素均未显著刺激RSC中2-脱氧-D-葡萄糖的摄取。用人重组白细胞介素-1β（IL-1β）处理NRSC可使葡萄糖摄取增加至与RSC基础摄取相似的水平。这些结果表明，RSC自分泌产生IL-1β可能是这些细胞基础葡萄糖摄取较高的原因。
