Lucarelli Adrienne Pratti, Martins Maria Marta, Montor Wagner, Oliveira Vilmar, Galvão Maria Antonieta Longo, Piato Sebastião
Department of Gynecology and Obstetrics, Santa Casa de Misericórdia de São Paulo Hospital, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, Brazil.
Sao Paulo Med J. 2011 Dec;129(6):371-9. doi: 10.1590/s1516-31802011000600002.
Cyclooxygenase-2 (COX-2) and human epidermal growth factor receptor type 2 (HER-2) are associated with tumorigenesis. Studies have shown that HER-2 can regulate COX-2 expression. The aim of this study was to evaluate the correlation between COX-2 and HER-2 expression in normal breast epithelium and in ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC) present in the same breast.
Cross-sectional study at the Mastology Unit of the Department of Gynecology and Obstetrics, Santa Casa de Misericórdia de São Paulo Hospital.
COX-2 and HER-2 were detected using immunohistochemistry on 100 tissue fragments. HER-2 > +2 was subjected to fluorescence in situ hybridization (FISH).
COX-2 expression was detected in 87%, 85% and 75% of IDC, DCIS and normal epithelium, respectively. HER-2 expression was detected in 34% of IDC and 34% of DCIS. COX-2 in DCIS correlated with HER-2 in IDC (P = 0.049) and DCIS (P = 0.049). COX-2 in normal epithelium correlated with HER-2 in IDC (P = 0.046) and DCIS (P = 0.046). COX-2 in IDC was not associated with HER-2 (P = 0.235). Comparison between COX-2 and HER-2 in DCIS showed that there was a statistically significant difference with regard to nuclear grades II and III and presence of comedonecrosis (P < 0.001). In IDC, there was significant expression with nuclear grades II and III and histological grade II (P < 0.001).
Our findings provide evidence that HER-2 and COX-2 regulate each other.
环氧化酶-2（COX-2）和人表皮生长因子受体2（HER-2）与肿瘤发生相关。研究表明，HER-2可调节COX-2的表达。本研究旨在评估COX-2与HER-2在同一乳腺的正常乳腺上皮、导管原位癌（DCIS）和浸润性导管癌（IDC）中的表达相关性。
在圣保罗慈悲圣母医院妇产科乳腺科进行的横断面研究。
采用免疫组织化学方法检测100个组织切片中的COX-2和HER-2。HER-2> +2的样本进行荧光原位杂交（FISH）检测。
COX-2在IDC、DCIS和正常上皮中的表达率分别为87%、85%和75%。HER-2在IDC和DCIS中的表达率均为34%。DCIS中的COX-2与IDC中的HER-2相关（P = 0.049），也与DCIS中的HER-2相关（P = 0.049）。正常上皮中的COX-2与IDC中的HER-2相关（P = 0.046），也与DCIS中的HER-2相关（P = 0.046）。IDC中的COX-2与HER-2不相关（P = 0.235）。DCIS中COX-2与HER-2的比较显示，在核分级II和III以及粉刺样坏死的存在方面存在统计学显著差异（P < 0.001）。在IDC中，核分级II和III以及组织学分级II时有显著表达（P < 0.001）。
我们的研究结果提供了证据表明HER-2和COX-2相互调节。
