光活化荧光探针及其在生物成像中的应用技术。
Photoactivatable fluorophores and techniques for biological imaging applications.
机构信息
Departments of Cell Biology and of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA.
出版信息
Photochem Photobiol Sci. 2012 Mar;11(3):460-71. doi: 10.1039/c2pp05342j. Epub 2012 Jan 17.
Abstract
Photoactivatable fluorophores (PAFs) are powerful imaging probes for tracking molecular and cellular dynamics with high spatiotemporal resolution in biological systems. Recent developments in biological microscopy have raised new demands for engineering new PAFs with improved properties, such as high two photon excitation efficiency, reversibility, cellular delivery and targeting. Here we review the history and some of the recent developments in this area, emphasizing our efforts in developing a new class of caged coumarins and related imaging methods for studying dynamic cell-cell communication through gap junction channels, and in extending the application of these caged coumarins to new areas including spatiotemporal control of microRNA activity in vivo.
摘要
光活化荧光团（PAFs）是一种强大的成像探针，可用于在生物系统中以高时空分辨率跟踪分子和细胞动态。近年来，生物学显微镜技术的发展对工程新型 PAFs 提出了新的要求，例如提高双光子激发效率、可逆性、细胞传递和靶向性等性能。本文回顾了该领域的历史和一些最新进展，重点介绍了我们在开发新型笼状香豆素和相关成像方法方面的工作，这些方法用于研究通过间隙连接通道的动态细胞间通讯，以及将这些笼状香豆素的应用扩展到新的领域，包括体内 microRNA 活性的时空控制。
相似文献
1
Photoactivatable fluorophores and techniques for biological imaging applications.光活化荧光探针及其在生物成像中的应用技术。
Photochem Photobiol Sci. 2012 Mar;11(3):460-71. doi: 10.1039/c2pp05342j. Epub 2012 Jan 17.
2
New caged coumarin fluorophores with extraordinary uncaging cross sections suitable for biological imaging applications.具有适用于生物成像应用的非凡光解笼效应截面的新型笼状香豆素荧光团。
J Am Chem Soc. 2004 Apr 14;126(14):4653-63. doi: 10.1021/ja036958m.
3
Development of Photoactivated Fluorescent N-Hydroxyoxindoles and Their Application for Cell-Selective Imaging.光活化荧光N-羟基吲哚的开发及其在细胞选择性成像中的应用。
Chemistry. 2016 Apr 25;22(18):6361-7. doi: 10.1002/chem.201600547. Epub 2016 Mar 23.
4
Photostable and photoswitching fluorescent dyes for super-resolution imaging.用于超分辨率成像的光稳定和光开关荧光染料。
J Biol Inorg Chem. 2017 Jul;22(5):639-652. doi: 10.1007/s00775-016-1435-y. Epub 2017 Jan 12.
5
Photoactivatable BODIPYs for Live-Cell PALM.用于活细胞 PALM 的光活化硼二吡咯亚甲基染料
Molecules. 2023 Mar 7;28(6):2447. doi: 10.3390/molecules28062447.
6
Ultrabright photoactivatable fluorophores created by reductive caging.通过还原笼蔽作用产生的超高亮光活化荧光团。
Nat Methods. 2012 Dec;9(12):1181-4. doi: 10.1038/nmeth.2214. Epub 2012 Oct 28.
7
Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Imaging.通过局部光激活和荧光成像追踪活细胞中的动态间隙连接耦合
Methods Mol Biol. 2016;1437:181-91. doi: 10.1007/978-1-4939-3664-9_13.
8
Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores.利用光活化有机荧光染料对固定和活细胞中的靶向蛋白进行超分辨率成像。
J Am Chem Soc. 2010 Nov 3;132(43):15099-101. doi: 10.1021/ja1044192.
9
Synthesis of photoactivatable azido-acyl caged oxazine fluorophores for live-cell imaging.用于活细胞成像的光可激活叠氮酰基笼形恶嗪荧光团的合成。
Chem Commun (Camb). 2016 Jul 19;52(60):9442-5. doi: 10.1039/c6cc04882j.
10
Site-Specific Incorporation of a Photoactivatable Fluorescent Amino Acid.位点特异性引入光活化荧光氨基酸。
Chembiochem. 2021 Feb 2;22(3):501-504. doi: 10.1002/cbic.202000602. Epub 2020 Nov 2.
引用本文的文献
1
Cilia dynamics create a dynamic barrier to penetration of the periciliary layer in human airway epithelia.纤毛动力学对人呼吸道上皮细胞周纤毛层的穿透形成了动态屏障。
Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2419032122. doi: 10.1073/pnas.2419032122. Epub 2025 Jul 11.
2
Hydroxy-pendant tetrazole as the cage group for photoactivatable push-pull fluorophores.羟基取代的四唑作为光可激活推拉型荧光团的笼状基团。
Chem Sci. 2025 Jun 24. doi: 10.1039/d5sc03279b.
3
Sulfur-tetrazine as highly efficient visible-light activatable photo-trigger for designing photoactivatable fluorescence biomolecules.硫代四嗪作为高效可见光激活光触发剂，用于设计光激活荧光生物分子。
J Mater Chem B. 2024 Oct 30;12(42):10839-10849. doi: 10.1039/d4tb01817f.
4
Photoactivatable Fluorophores for Bioimaging Applications.用于生物成像应用的光可激活荧光团。
ACS Appl Opt Mater. 2023 Mar 24;1(3):640-651. doi: 10.1021/acsaom.3c00025. Epub 2023 Feb 27.
5
Photoactivatable BODIPYs for Live-Cell PALM.用于活细胞 PALM 的光活化硼二吡咯亚甲基染料
Molecules. 2023 Mar 7;28(6):2447. doi: 10.3390/molecules28062447.
6
Multifunctional stimuli-responsive chemogenetic platform for conditional multicolor cell-selective labeling.用于条件性多色细胞选择性标记的多功能刺激响应化学遗传平台。
Chem Sci. 2022 Oct 3;13(41):12187-12197. doi: 10.1039/d2sc03100k. eCollection 2022 Oct 26.
7
The Development and Application of Opto-Chemical Tools in the Zebrafish.光学化学工具在斑马鱼中的发展与应用。
Molecules. 2022 Sep 22;27(19):6231. doi: 10.3390/molecules27196231.
8
Optical writing and single molecule reading of photoactivatable and silver nanoparticle-enhanced fluorescence.光可激活及银纳米颗粒增强荧光的光学写入与单分子读取
Nanoscale Adv. 2020 Feb 28;2(5):1956-1966. doi: 10.1039/d0na00049c. eCollection 2020 May 19.
9
Oxime as a general photocage for the design of visible light photo-activatable fluorophores.肟作为用于设计可见光光激活荧光团的通用光笼。
Chem Sci. 2021 Nov 22;12(47):15572-15580. doi: 10.1039/d1sc05351e. eCollection 2021 Dec 8.
10
Flipping the Switch: Innovations in Inducible Probes for Protein Profiling.翻转开关：蛋白质分析诱导探针的创新。
ACS Chem Biol. 2021 Dec 17;16(12):2719-2730. doi: 10.1021/acschembio.1c00572. Epub 2021 Nov 15.
本文引用的文献
1
Temporal and spatial regulation of microRNA activity with photoactivatable cantimirs.光激活 cantimirs 调控 microRNA 活性的时空特异性
ACS Chem Biol. 2011 Dec 16;6(12):1332-8. doi: 10.1021/cb200290e. Epub 2011 Oct 11.
2
Proteins on the move: insights gained from fluorescent protein technologies.蛋白质的运动：荧光蛋白技术的新发现。
Nat Rev Mol Cell Biol. 2011 Sep 23;12(10):656-68. doi: 10.1038/nrm3199.
3
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP.利用光致变色 GFP 实现无衍射极限的全光学成象和写入。
Nature. 2011 Sep 11;478(7368):204-8. doi: 10.1038/nature10497.
4
A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching.一种可光开关的类 GFP 蛋白，其荧光激发与开关解耦。
Nat Biotechnol. 2011 Sep 11;29(10):942-7. doi: 10.1038/nbt.1952.
5
Intercellular bridges in vertebrate gastrulation.脊椎动物原肠胚形成中的细胞间桥。
PLoS One. 2011;6(5):e20230. doi: 10.1371/journal.pone.0020230. Epub 2011 May 25.
6
Imaging of Pressure- and Electrokinetically Driven Flows through Open Capillaries.通过开放毛细管的压力驱动流和电动力学驱动流的成像
Anal Chem. 1998 Jul 1;70(13):2459-67. doi: 10.1021/ac9709662.
7
How to obtain labeled proteins and what to do with them.如何获取标记蛋白以及如何处理它们。
Curr Opin Biotechnol. 2010 Dec;21(6):766-76. doi: 10.1016/j.copbio.2010.09.011. Epub 2010 Oct 26.
8
Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores.利用光活化有机荧光染料对固定和活细胞中的靶向蛋白进行超分辨率成像。
J Am Chem Soc. 2010 Nov 3;132(43):15099-101. doi: 10.1021/ja1044192.
9
Small photoactivatable molecules for controlled fluorescence activation in living cells.用于活细胞中荧光激活控制的小型光活化分子。
Bioorg Med Chem. 2011 Feb 1;19(3):1023-9. doi: 10.1016/j.bmc.2010.07.011. Epub 2010 Jul 31.
10
Rational design, synthesis, and characterization of highly fluorescent optical switches for high-contrast optical lock-in detection (OLID) imaging microscopy in living cells.理性设计、合成及高荧光光学开关的特性，用于活细胞的高对比度光学锁定检测（OLID）成像显微镜。
Bioorg Med Chem. 2011 Feb 1;19(3):1030-40. doi: 10.1016/j.bmc.2010.07.015. Epub 2010 Jul 30.
Zotero 插件