Department of Chemistry, Stanford University, Stanford, California 94305, USA.
J Am Chem Soc. 2010 Nov 3;132(43):15099-101. doi: 10.1021/ja1044192.
Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push-pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.
基于单分子稀疏子集的顺序成像的超分辨率成像技术需要荧光团，其发射可以光活化或光切换。因为典型的有机荧光团可以发射比平均荧光蛋白多得多的光子，所以有机荧光团在超分辨率成像方案中有潜在的优势，但必须提供针对特定细胞蛋白的靶向。我们报告了基于 HaloTag 的靶向叠氮 DCDHF 的设计和应用，这是一类光活化的推拉式荧光团，可产生适合活细菌和固定哺乳动物细胞中单分子超分辨率成像的明亮荧光标记。
