Katagiri H, Okayasu I, Endo M
First Department of Surgery, Faculty of Medicine, Tokyo Medical and Dental University.
Bull Tokyo Med Dent Univ. 1990 Jun;37(1-2):1-18.
The nuclear DNA content in 71 cases of periampullary cancer (27, cancer of the head of the pancreas (Ph); 24, cancer of the ampulla of Vater (A); and 20, cancer of the inferior common bile duct (Bi] was measured cytofluorometrically using the archival paraffin-embedded specimens of the primary lesions. They were analyzed in relation to prognosis, tumor size, histological differentiation, lymph node metastasis, lymphatic invasion, venous invasion, perineural invasion, and growth pattern. The results show that "Ph" has more unfavorable prognosis compared with the other two and it has more DNA content under the same conditions such as tumor from 2.1 to 4 cm in its greatest dimension, well differentiated adenocarcinoma, with or without lymph node metastasis, with or without venous invasion, with lymphatic invasion, with perineural invasion, and in the intermediate growth pattern between expansive and infiltrative. In conclusion, this study demonstrates a close correlation between the DNA content and prognosis and the significant clinical value of DNA analysis for predicting the prognosis in patients of periampullary cancer.
利用原发性病变的存档石蜡包埋标本，通过细胞荧光光度法测量了71例壶腹周围癌（27例胰腺头部癌（Ph）；24例 Vater 壶腹癌（A）；20例胆总管下段癌（Bi））的核DNA含量。对其与预后、肿瘤大小、组织学分化、淋巴结转移、淋巴管浸润、静脉浸润、神经周围浸润和生长方式进行了分析。结果显示，与其他两者相比，“Ph”的预后更差，并且在相同条件下，如肿瘤最大直径为2.1至4厘米、高分化腺癌、有无淋巴结转移、有无静脉浸润、有淋巴管浸润、有神经周围浸润以及处于膨胀性和浸润性之间的中间生长方式时，“Ph”的DNA含量更高。总之，本研究表明DNA含量与预后之间存在密切相关性，以及DNA分析对预测壶腹周围癌患者预后具有重要的临床价值。
