Li Yiyan, Tian Xulong, Qian Long, Yu Xuehong, Jiang Weiwei
Bioengineering College, Chongqing University, Chongqing 400030, China.
Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:765-8. doi: 10.1109/IEMBS.2011.6090175.
The unilaterally lesioned rat model of Parkinson's disease which fails to orient to the food stimuli presented on the contralateral side of its preferential side of body could be induced by the injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle (MFB). We employed transcranial direct current stimulation (tDCS, current intensity: 80 μA, and 40 μA; anodal electrode area: 3.14 mm(2); stimulation time: 30 minutes) over the M1 area to relieve the ipsilateral bias in the rat model. A corridor test was set to count the ipsilateral bias of the rats. In this experiment, 30 Sprague-Dawley rats (80 μA: n = 8, 40 μA: n = 8, sham: n = 7, healthy control: n = 7) were chosen for the corridor test and the tDCS session. The lesioned rats exhibited increased ipsilateral bias 4 weeks after the lesion surgery (P < 0.01), and the anodal tDCS with the active electrode on the lesioned side relieved the ipsilateral bias significantly (P < 0.01) immediately after the surgery and the improvement lasted for nearly 1 day. The rats in the group of 80 μA exhibited more significant changes than the 40 μA group after one day. After all the experiments, the histological process showed no neurotrauma led by the tDCS. In conclusion, the modulatory function of the cortical excitability of the tDCS may awaken the compensatory mechanisms and the response mechanisms which modulate the loss of the brain function. Further studies should be done to provide more evidence about the assumption.
通过向内侧前脑束（MFB）注射6-羟基多巴胺（6-OHDA），可以诱导出帕金森病单侧损伤大鼠模型，该模型无法朝向其偏好身体侧对侧呈现的食物刺激定向。我们采用经颅直流电刺激（tDCS，电流强度：80 μA和40 μA；阳极电极面积：3.14 mm(2)；刺激时间：30分钟）作用于M1区域，以减轻大鼠模型中的同侧偏向。设置了走廊试验来计算大鼠的同侧偏向。在本实验中，选择30只Sprague-Dawley大鼠（80 μA组：n = 8，40 μA组：n = 8，假手术组：n = 7，健康对照组：n = 7）进行走廊试验和tDCS治疗。损伤大鼠在损伤手术后4周同侧偏向增加（P < 0.01），且阳极tDCS将活性电极置于损伤侧在手术后立即显著减轻了同侧偏向（P < 0.01），且改善持续了近1天。1天后，80 μA组大鼠的变化比40 μA组更显著。所有实验结束后，组织学检查显示tDCS未导致神经创伤。总之，tDCS对皮质兴奋性的调节功能可能会唤醒调节脑功能丧失的代偿机制和反应机制。应进行进一步研究以提供更多关于该假设的证据。
