Prasad Abhishek, Sankar Viswanath, Dyer Aubrey T, Knott Eric, Xue Qing-Shan, Nishida Toshikazu, Reynolds John R, Shaw Gerry, Streit Wolfgang, Sanchez Justin C
University of Miami, Coral Gables, FL 33146, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:3020-3. doi: 10.1109/IEMBS.2011.6090827.
In this work, we develop an experimental testbed that couples biotic and abiotic metrics for studying, quantifying and predicting the effects of chronic electrode implantation on neural electrode performance. The rationale is based on the observation that long-term functionality is the outcome of the interactions between the dynamics of the neuronal environment and the properties of the electrode itself. By combining and analyzing the substantially richer information available in the spatiotemporal dynamics of neurons with biotic and abiotic metrics such as biochemical markers, histochemistry, SEM imaging, and electrochemistry, we seek to quantitatively improve our understanding of the functional modifications underlying the long-term responses of electrode implants. The goal is to ultimately enable the design of future reliable interfaces. In our preliminary analysis using this biotic-abiotic approach of an electrode 18 days post-implant, we observed both structural and histochemical responses related to chronic electrode implantation. These were coupled to daily functional changes in electrode performance. Interestingly, these changes were not correlated with markers of brain injury at the time of electrode explantation. Future work using this multidisciplinary approach is directed to providing a detailed perspective into long-term microelectrode performance.
在这项工作中，我们开发了一个实验平台，该平台结合了生物和非生物指标，用于研究、量化和预测慢性电极植入对神经电极性能的影响。其基本原理基于这样的观察结果：长期功能是神经元环境动态与电极本身特性之间相互作用的结果。通过将神经元时空动态中可用的丰富得多的信息与生物化学标记、组织化学、扫描电子显微镜成像和电化学等生物和非生物指标相结合并进行分析，我们试图定量地增进对电极植入物长期反应背后功能改变的理解。目标是最终实现未来可靠接口的设计。在我们使用这种生物 - 非生物方法对植入后18天的电极进行的初步分析中，我们观察到了与慢性电极植入相关的结构和组织化学反应。这些反应与电极性能的日常功能变化相关联。有趣的是，这些变化与电极取出时的脑损伤标记物无关。使用这种多学科方法的未来工作旨在提供对长期微电极性能的详细见解。
