Umetani Keiji, Pearson James T, Schwenke Daryl O, Shirai Mikiyasu
Japan Synchrotron Radiation Research Institute, Sayo-gun, Hyogo 679-5198, Japan.
Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:7791-4. doi: 10.1109/IEMBS.2011.6091920.
This study elucidates the vascular internal diameter response of coronary arterial circulation in closed-chest rats to evaluate endothelium-dependent and endothelium-independent vasodilatory ability and to investigate disease mechanisms. For this study, we developed an X-ray intravital microscopy system using a microangiography technique and a synchrotron radiation source at SPring-8. An X-ray direct-conversion type detector with 7-μm spatial resolution was used for real-time imaging. Microangiographic images were stored in a digital frame memory system at a maximum rate of 30 frame/s with a 1024 × 1024-pixel, 10-bit format. In imaging experiments, the small coronary arteries were visualized after iodine contrast agent injection into the coronary artery.
本研究阐明了闭胸大鼠冠状动脉循环的血管内径反应，以评估内皮依赖性和非内皮依赖性血管舒张能力，并研究疾病机制。在本研究中，我们利用微血管造影技术和SPring-8的同步辐射源开发了一种X射线活体显微镜系统。使用具有7μm空间分辨率的X射线直接转换型探测器进行实时成像。微血管造影图像以1024×1024像素、10位格式、最高30帧/秒的速率存储在数字帧存储系统中。在成像实验中，向冠状动脉注射碘造影剂后可观察到小冠状动脉。
