一种用于离体心脏组织电生理特性表征的廉价替代浴槽系统。
An inexpensive alternative bath system for electrophysiological characterization of isolated cardiac tissue.
作者信息
Villa Emanuel, White Alayne Stewart, Liao Jun, de Jongh Curry Amy L
机构信息
Joint Graduate Program inBiomedical Engineering, The University of Memphis and University of Tennessee Health Science Center, Memphis, TN 38152, USA.
出版信息
Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:8424-7. doi: 10.1109/IEMBS.2011.6092078.
A tissue bath system, to be used as an alternative to complex perfusion chambers, was constructed for use in cardiac electrophysiological studies. This system consists of an acrylic chamber to hold circulating physiological medium such as DMEM, suspended in a water bath warmed by a hot plate. Temperature and pH were controlled to mimic physiological conditions. Rat and porcine cardiac tissues, were used to test viability of the conditions presented in the bath system. Using a cardiac mapping system, the tissues were stimulated and responses recorded. From the recordings we were able to calculate conduction velocities and spatial dispersion of activation indices. The results are comparable to previous in-vivo work, which suggests that the tissue bath system design can maintain tissue viability. This tissue bath system is a relatively simple alternative for ex-vivo testing of cardiac tissues.
构建了一种组织浴系统，作为复杂灌注室的替代方案，用于心脏电生理研究。该系统由一个丙烯酸腔室组成，用于容纳循环的生理介质，如DMEM，悬浮在由热板加热的水浴中。温度和pH值被控制以模拟生理条件。使用大鼠和猪的心脏组织来测试浴系统中所呈现条件的可行性。使用心脏标测系统对组织进行刺激并记录反应。从记录中我们能够计算传导速度和激活指数的空间离散度。结果与之前的体内研究相当，这表明组织浴系统设计能够维持组织的活力。这种组织浴系统是用于心脏组织体外测试的一种相对简单的替代方案。
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