γ干扰素释放试验用于检测肺结核病例的偶然和密切接触者中的潜伏性结核感染。

Interferon-gamma release assay for detection of latent tuberculosis infection in casual and close contacts of tuberculosis cases.

机构信息

Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Zagazig, Zagazig, Egypt.

出版信息

East Mediterr Health J. 2011 Oct;17(10):749-53. doi: 10.26719/2011.17.10.749.

DOI:10.26719/2011.17.10.749

Abstract

The tuberculin skin test (TST) has many limitations for the diagnosis of latent tuberculosis (TB infection. The aim of this study in Egypt was to estimate the usefulness of an interferon-gamma release assay (IFN-gamma) assay for the detection of latent TB infection in contacts of active TB cases. A total of 116 participants were enrolled and divided into 3 groups: community controls, casual (laboratory and clinic) contacts and close (household) contacts. Subjects diagnosed with latent TB infection by TST were 11.5% of controls, 71.1% of casual contacts and 29.6% of close contacts. Subjects diagnosed as latent TB infection by IFN-gamma assay (QuantiFERON-TB Gold In-Tube) were 5.9% of controls, 31.0% of casual contacts and 33.3% of close contacts. The overall agreement between TST and IFN-gamma was 66.7% (kappa = 0.28). The IFN-gamma method could be more helpful than TST for detection of latent TB infection in contacts.

摘要

结核菌素皮肤试验（TST）在诊断潜伏性结核（TB 感染方面存在诸多局限性。本研究在埃及的目的是评估干扰素-γ释放试验（IFN-γ）检测活动性 TB 病例接触者潜伏性 TB 感染的有效性。共纳入 116 名参与者，并分为 3 组：社区对照者、偶然（实验室和诊所）接触者和密切（家庭）接触者。TST 诊断为潜伏性 TB 感染者的比例分别为对照组的 11.5%、偶然接触者的 71.1%和密切接触者的 29.6%。IFN-γ检测（QuantiFERON-TB Gold In-Tube）诊断为潜伏性 TB 感染者的比例分别为对照组的 5.9%、偶然接触者的 31.0%和密切接触者的 33.3%。TST 和 IFN-γ之间的总体一致性为 66.7%（kappa = 0.28）。IFN-γ 方法在检测潜伏性 TB 感染方面可能比 TST 更有帮助。