Yu Fulai, Fang Yuqiang, Wang Wenquan, Wang Qiuling, Liu Fengbo
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(18):2457-61.
The main aim of the research was to evaluate genetic variability and interrelationships of mainly quantitative traits in 2-year population, and provide a basis for high-yield breeding of Glycyrrhiza uralensis.
Four genotype G. uralensis population were transplanting in four different environment using complete randomized block design with three replication, and the 10 quantitative traits, including plant height (PH), stem diameter (SD), tiller number (TN), taproot length (TRL), root length (RL), root diameter (RD), diameter of 20 cm below the root head (D20), taperingness (TR), lateral root number (LRN) and root fresh weight (RFW) were measured in field.
The difference among population for all evaluated traits were significant (P<0.05) through Duncan's multiple range tests, and the coefficient of variation of RFW and LRN were above 25%. The analysis of variance was used to evaluate the traits of four populations across to four different environment Genotype, environment and their interaction effect were significant (P<0.05) or highly significant (P<0.01) for mainly evaluated traits. Simple correlation between traits showed that PH, SD, LRN, RL, RD and D20 had highly significant (P<0.01) and positive correlation with RFW. Results of the path coefficient analyses showed that D20 had the greatest positive direct effect on RFW, followed by the traits of PH and RL.
Selection for increased D20, RL and PH would be the best indirect selection traits for increasing root yield. Meanwhile, ample genetic variability exists in the G. uralensis 2-year population, it could be used for breeding improvement of root yield.
本研究的主要目的是评估两年生群体中主要数量性状的遗传变异性和相互关系,为乌拉尔甘草的高产育种提供依据。
采用完全随机区组设计,将4个基因型的乌拉尔甘草群体移栽到4种不同环境中,重复3次,在田间测定株高(PH)、茎粗(SD)、分蘖数(TN)、主根长度(TRL)、根长(RL)、根直径(RD)、根头以下20cm处直径(D20)、根的尖削度(TR)、侧根数(LRN)和根鲜重(RFW)等10个数量性状。
通过邓肯多重极差检验,所有评估性状在群体间的差异均显著(P<0.05),根鲜重和侧根数的变异系数均高于25%。采用方差分析评估4个群体在4种不同环境下的性状,基因型、环境及其互作效应在主要评估性状上显著(P<0.05)或极显著(P<0.01)。性状间的简单相关分析表明,株高、茎粗、侧根数、根长、根直径和根头以下20cm处直径与根鲜重呈极显著(P<0.01)正相关。通径系数分析结果表明,根头以下20cm处直径对根鲜重的正向直接效应最大,其次是株高和根长性状。
选择增加根头以下20cm处直径、根长和株高将是提高根产量的最佳间接选择性状。同时,乌拉尔甘草两年生群体存在丰富的遗传变异,可用于根产量的育种改良。