Wei Shengli, Wang Wenquan, Liu Changli, Wang Jiyong, Zhang Ruifeng, Sun Minbin
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2012 Mar;37(5):553-7.
To estimate the broad-sense heritability of the production of Glycyrrhiza uralensis and the content of glycyrrhizin as well as the genetic relationship of various growth indexes and biomass indexes, and provide the scientific basis for establishment of high quality licorice cultivate technology system.
The randomized method was used to assign the provenance trial, the content of glycyrrhizin was determined by HPLC, and the method of classic genetics was applied to estimate the broad-sense heritability and genetic correlation coefficient.
The content of glycyrrhizin is influenced by the growth environment and gene, but the growth environment is the dominant factor. The estimated result of single sites about broad-sense heritability (h2) showed that the production of G. uralensis (W(u)) and the content of glycyrrhizin was controlled by gene which the broad-sense heritability was 0.663 2, 0.751 1 respectively, they had some potential on genetic modification. The results of genetic analysis correlation showed that the plant height and the stem diameter was positive (P < 0.01) correlated significantly with the production (W(u)) either on phenotype or on genetic, it suggests that the plant height and the stem diameter could be the index above ground to assessment the production of the G. uralensis. The content of glycyrrhizin had a positive correlation with the number of lateral root (P < 0.05), but it had a negative correlation with the plant height, stem diameter, diameter of root top (D(r)), the total biomass (W(t)) and the biomass underground (W(u)) on inheritance. It is suggested that it was difficult to achieve both high content and high yield simultaneously in the genetic improvement, so we should have a deeply thought about the specific improvement target when making the reformed scheme.
估算乌拉尔甘草产量及甘草酸含量的广义遗传力，以及各生长指标和生物量指标间的遗传关系，为建立优质甘草栽培技术体系提供科学依据。
采用随机区组法进行种源试验，用高效液相色谱法测定甘草酸含量，应用经典遗传学方法估算广义遗传力和遗传相关系数。
甘草酸含量受生长环境和基因的影响，但生长环境是主导因素。单地点广义遗传力（h2）估算结果表明，乌拉尔甘草产量（W(u)）和甘草酸含量受基因控制，广义遗传力分别为0.663 2、0.751 1，具有一定的遗传改良潜力。遗传相关分析结果表明，株高和茎粗无论是表型还是遗传上均与产量（W(u)）呈极显著正相关（P < 0.01），表明株高和茎粗可作为地上部分评估乌拉尔甘草产量的指标。甘草酸含量与侧根数呈正相关（P < 0.05），但在遗传上与株高、茎粗、根头直径（D(r)）、总生物量（W(t)）和地下生物量（W(u)）呈负相关。表明在遗传改良中难以同时实现高含量和高产，因此在制定改良方案时应深入思考具体的改良目标。
