Tao Jian, Yang Jing, Chen Chaoyin, Zhao Shenglan, Ben Kunlong
Kunming University, Kunming 650214, China.
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(18):2548-51.
To study anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro.
Anti-HIV-1 activity was detected with syncytial formation assay and quantitative P24 enzyme-linked immunosorbent assay (ELISA); cytotoxicity was tested with MTT colorimetric assay. Antiviral mechanism was investigated by fusion inhibition, time of addition and pre-treatment experiments.
The 50% inhibition concentrations (IC50) of PS20 for HIV-1(IIIB), HIV-1(Ada-M), and HIV-1(Bal), were 0.26, 0.46, 0.90 micromol x L(-1), respectively. Studies on antiviral mechanism of PS20 showed that target molecule may be viral envelope protein.
The results suggested that PS20 had high anti-HIV activity and was worth to be studied further.
研究隔山消硫酸葡聚糖体外抗HIV活性及作用机制。
采用合胞体形成试验和定量P24酶联免疫吸附测定法（ELISA）检测抗HIV-1活性；采用MTT比色法检测细胞毒性。通过融合抑制、加药时间和预处理实验研究抗病毒机制。
PS20对HIV-1（IIIB）、HIV-1（Ada-M）和HIV-1（Bal）的50%抑制浓度（IC50）分别为0.26、0.46、0.90微摩尔·升（-1）。PS20抗病毒机制研究表明，靶分子可能是病毒包膜蛋白。
结果提示PS20具有较高的抗HIV活性，值得进一步研究。
