State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, China; University of the Chinese Academy of Sciences, Beijing, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Fitoterapia. 2019 Jul;136:104171. doi: 10.1016/j.fitote.2019.104171. Epub 2019 May 11.
Seven undescribed C21 steroids, namely cynanchin A-G, together with thirteen known analogues, were isolated from the roots of cynanchum otophyllum. Their structures were elucidated by 1D, 2D NMR and MS spectra, as well as chemical methods. Meanwhile, all of isolates were tested for their anti-hepatic fibrosis activity. Among them, compounds 4-6, 10-12 and 14-17 showed moderate or significant inhibitory effects for the proliferation of hepatic stellate cells (HSCs) induced by transforming growth factor-β1 (TGF-β1) in vitro.
从鹅绒藤根部分离得到了七个未被描述的 C21 甾体，分别为 Cynanchin A-G，以及十三个已知类似物。通过 1D、2D NMR 和 MS 谱以及化学方法确定了它们的结构。同时，对所有分离物进行了抗肝纤维化活性测试。其中，化合物 4-6、10-12 和 14-17 对转化生长因子-β1（TGF-β1）诱导的肝星状细胞（HSCs）增殖具有中度或显著的抑制作用。
