Research and Inspection Center of Traditional Chinese Medicine and Ethnomedicine, National Institutes for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
J Pharm Biomed Anal. 2012 Mar 25;62:191-5. doi: 10.1016/j.jpba.2011.12.024. Epub 2011 Dec 29.
An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC/Q-TOF-MS) method coupled with a principal component analysis (PCA) was developed and applied toward identifying donkey-hide gelatin, bovine-hide gelatin, pig-hide gelatin, tortoise shell glue, and deerhorn glue. The UPLC-MS data of the trypsin digested samples were subjected to principal component analysis (PCA) in order to classify these five gelatins. Additionally, marker peptides given by the loadings plot of PCA were identified based on a comparison of recorded LC-MS data with a previously reported database of the corresponding gelatin variants. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of gelatins commonly used in Traditional Chinese Medicine (TCM).
建立了一种超高效液相色谱-四极杆飞行时间质谱（UPLC/Q-TOF-MS）联用主成分分析（PCA）的方法，用于鉴定驴皮明胶、牛皮明胶、猪皮明胶、龟甲胶和鹿角胶。对胰蛋白酶消化样品的 UPLC-MS 数据进行主成分分析（PCA），以对这五种明胶进行分类。此外，根据 PCA 加载图提供的标记肽，通过与先前报道的相应明胶变体数据库进行比较，对记录的 LC-MS 数据进行鉴定。本研究结果表明，该方法可靠，已成功应用于中药（TCM）中常用明胶变体的鉴定。
