Ma Jieyao, Li Kailin, Shi Silin, Li Jian, Tang Sunv, Liu LiangHong
School of Pharmaceutical Sciences, Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, Hunan University of Medicine, Huaihua, China.
Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua, China.
Front Pharmacol. 2022 Jun 2;13:922488. doi: 10.3389/fphar.2022.922488. eCollection 2022.
UHPLC-HRMS (ultra-high-performance liquid chromatography-high resolution mass spectrometry) is a new technique that unifies the application of UHPLC with HRMS. Because of the high sensitivity and good separation ability of UHPLC and the sensitivity of HRMS, this technique has been widely used for structure identification, quantitative determination, fingerprint analysis, and elucidation of the mechanisms of action of traditional Chinese medicines (TCMs) in recent years. This review mainly outlines the advantages of using UHPLC-HRMS and provides a survey of the research advances on UHPLC-HRMS for the quality control of TCMs.
超高效液相色谱-高分辨率质谱联用(UHPLC-HRMS)是一种将超高效液相色谱(UHPLC)与高分辨率质谱(HRMS)联用的新技术。由于UHPLC具有高灵敏度和良好的分离能力,以及HRMS的高灵敏度,近年来该技术已广泛应用于中药的结构鉴定、定量测定、指纹图谱分析和作用机制阐释。本综述主要概述了使用UHPLC-HRMS的优势,并对UHPLC-HRMS在中药质量控制方面的研究进展进行了综述。