Hardianto Ari, Kusumaningtyas Pintaka, Prabowo Sulistyo, Hartati Yeni Wahyuni
Doctoral Program in Analytical Chemistry, FMIPA Universitas Padjadjaran, Bandung, 45363, Indonesia.
Chemistry Education Study Program, Faculty of Teacher Training and Education, Universitas Mulawarman Samarinda, 75119, Indonesia.
Heliyon. 2023 Mar 8;9(3):e14418. doi: 10.1016/j.heliyon.2023.e14418. eCollection 2023 Mar.
Determination of halal food is essential in ensuring the tranquillity of consumers, especially Muslims. Halal products mean they are free from prohibited ingredients according to Islamic law. One ingredient that is prohibited is food products containing pork and its derivatives. An accurate verification method with a fast result is necessary to meet this requirement for halal food. DNA quantification of pork is now believed to be able to make accurate and quick decisions, as DNA acts as a reservoir or biological characterization of all living things, including pigs, according to specific characteristics of molecular and connection settings. Various DNA-based methods developed include PCR, biosensor and CRISPR methods. This review discussed various DNA-based Keywords: biosensor, CRISPR, detection, DNA, pork, PCR methods, including PCR, biosensor and CRISPR, to detect pork content in food. Among these methods, CRISPR is considered the easiest, fastest and most accurate. Therefore, it is important to develop this method further in the future. In this article, we provide a short review on DNA-based methods for detection of pork content in food products.
确定清真食品至关重要，这对于确保消费者尤其是穆斯林的安心至关重要。清真产品意味着它们不含有伊斯兰法律规定的违禁成分。一种被禁止的成分是含有猪肉及其衍生物的食品。需要一种结果快速的准确验证方法来满足对清真食品的这一要求。目前认为猪肉的DNA定量能够做出准确而快速的判断，因为根据分子和连接设置的特定特征，DNA充当了所有生物（包括猪）的储存库或生物特征。已开发的各种基于DNA的方法包括PCR、生物传感器和CRISPR方法。本综述讨论了各种基于DNA的方法，包括PCR、生物传感器和CRISPR，以检测食品中的猪肉含量。在这些方法中，CRISPR被认为是最简单、最快且最准确的。因此，未来进一步开发这种方法很重要。在本文中，我们对基于DNA的食品中猪肉含量检测方法进行了简要综述。
