绿色到红色光可转换的mEosFP辅助植物活体成像。

Green-to-red photoconvertible mEosFP-aided live imaging in plants.

作者信息

Mathur Jaideep, Griffiths Sarah, Barton Kiah, Schattat Martin H

机构信息

Department of Molecular and Cellular Biology, Laboratory of Plant Development and Interactions, University of Guelph, Guelph, Canada.

出版信息

Methods Enzymol. 2012;504:163-81. doi: 10.1016/B978-0-12-391857-4.00008-2.

DOI:10.1016/B978-0-12-391857-4.00008-2

PMID:22264534

Abstract

Numerous subcellular-targeted probes have been created using a monomeric green-to-red photoconvertible Eos fluorescent protein for understanding the growth and development of plants. These probes can be used to create color-based differentiation between similar cells, differentially label organelle subpopulations, and track subcellular structures and their interactions. Both green and red fluorescent forms of mEosFP are stable and compatible with single colored FPs. Differential highlighting using mEosFP probes greatly increases spatiotemporal precision during live imaging.

摘要

利用单体绿色到红色光转换Eos荧光蛋白已经构建了许多亚细胞靶向探针，用于了解植物的生长和发育。这些探针可用于在相似细胞之间进行基于颜色的区分、对细胞器亚群进行差异标记，以及追踪亚细胞结构及其相互作用。mEosFP的绿色和红色荧光形式都很稳定，并且与单色荧光蛋白兼容。使用mEosFP探针进行差异标记可大大提高实时成像过程中的时空精度。