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一种可视化细胞增殖的方法：以根尖分生组织为例

A Method to Visualize Cell Proliferation of : A Case Study of the Root Apical Meristem.

作者信息

Reyes-Olalde J Irepan, Tapia-Rodríguez Miguel, Pérez-Koldenkova Vadim, Contreras-Jiménez Gastón, Hernández-Herrera Paul, Corkidi Gabriel, Arciniega-González Arturo J, De La Paz-Sánchez Maria, García-Ponce Berenice, Garay-Arroyo Adriana, Álvarez-Buylla Elena R

机构信息

Laboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología Universidad Nacional Autónoma de México, Ciudad Universitaria Ciudad de México México.

Laboratorio de Botanica Universidad Estatal del Valle de Toluca Ocoyoacac Mexico.

出版信息

Plant Direct. 2025 Apr 28;9(4):e70060. doi: 10.1002/pld3.70060. eCollection 2025 Apr.

DOI:10.1002/pld3.70060
PMID:40297840
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12037192/
Abstract

Plant growth and development rely on a delicate balance between cell proliferation and cell differentiation. The root apical meristem (RAM) of is an excellent model to study the cell cycle due to the coordinated relationship between nucleus shape and cell size at each stage, allowing for precise estimation of the cell cycle duration. In this study, we present a method for high-resolution visualization of RAM cells. This is the first protocol that allows for simultaneous high-resolution imaging of cellular and nuclear stains, being compatible with DNA replication markers such as EdU, including fluorescent proteins (H2B::YFP), SYTOX DNA stains, and the cell wall stain SR2200. This protocol includes a clarification procedure that enables the acquisition of high-resolution 3D images, suitable for detailed subsequent analysis.

摘要

植物的生长和发育依赖于细胞增殖与细胞分化之间的微妙平衡。由于根顶端分生组织（RAM）在每个阶段细胞核形状与细胞大小之间存在协调关系，这使得能够精确估计细胞周期持续时间，因此它是研究细胞周期的一个出色模型。在本研究中，我们提出了一种用于高分辨率可视化RAM细胞的方法。这是首个允许对细胞和细胞核染色进行同步高分辨率成像的方案，它与诸如EdU等DNA复制标记兼容，包括荧光蛋白（H2B::YFP）、SYTOX DNA染色剂以及细胞壁染色剂SR2200。该方案包括一个澄清程序，可实现高分辨率三维图像的采集，适用于后续的详细分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/a878c90b9e47/PLD3-9-e70060-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/f18c0e1ec598/PLD3-9-e70060-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/fb80ea5b7e6b/PLD3-9-e70060-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/a1e38045a653/PLD3-9-e70060-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/f9a9f762587d/PLD3-9-e70060-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/2bf964ab701c/PLD3-9-e70060-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/dacfd2988075/PLD3-9-e70060-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/fbe4f190009b/PLD3-9-e70060-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/a878c90b9e47/PLD3-9-e70060-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/f18c0e1ec598/PLD3-9-e70060-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/fb80ea5b7e6b/PLD3-9-e70060-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/a1e38045a653/PLD3-9-e70060-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/f9a9f762587d/PLD3-9-e70060-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/2bf964ab701c/PLD3-9-e70060-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/dacfd2988075/PLD3-9-e70060-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/fbe4f190009b/PLD3-9-e70060-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce1c/12037192/a878c90b9e47/PLD3-9-e70060-g001.jpg

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本文引用的文献

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Arabidopsis Root Development Regulation by the Endogenous Folate Precursor, Para-Aminobenzoic Acid, via Modulation of the Root Cell Cycle.
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4
Nucleus size and its effect on nucleosome stability in living cells.细胞核大小及其对活细胞核小体稳定性的影响。
Biophys J. 2022 Nov 1;121(21):4189-4204. doi: 10.1016/j.bpj.2022.09.019. Epub 2022 Sep 21.
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Using positional information to provide context for biological image analysis with MorphoGraphX 2.0.利用位置信息为 MorphoGraphX 2.0 提供生物学图像分析的上下文。
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