Mironov Alexander A, Beznoussenko Galina V
Istituto FIRC di Oncologia Molecolare, Milan, Italy.
Methods Enzymol. 2012;504:201-19. doi: 10.1016/B978-0-12-391857-4.00010-0.
In biology, light microscopy (LM) is usually used to study phenomena at a global scale and to look for unique or rare events, and it also provides an opportunity for live imaging, while the forte of electron microscopy (EM) is the high resolution. Observation of living cells under EM is still impossible. Traditionally, LM and EM observations are carried out in different populations of cells/tissues. The advent of true correlative light-electron microscopy (CLEM) has allowed high-resolution imaging by EM of the very same structure observed by LM. This chapter describes imaging with the help of CLEM. The guidelines presented herein enable researchers to analyze structure of organelles and in particular rare events captured by low-resolution imaging of a population or transient events captured by live imaging can now also be studied at high resolution by EM.
在生物学中，光学显微镜（LM）通常用于在全局尺度上研究现象并寻找独特或罕见事件，它还提供了活细胞成像的机会，而电子显微镜（EM）的优势在于高分辨率。在电子显微镜下观察活细胞仍然是不可能的。传统上，光学显微镜和电子显微镜观察是在不同的细胞/组织群体中进行的。真正的 correlative 光电子显微镜（CLEM）的出现使得能够对光学显微镜观察到的相同结构进行电子显微镜高分辨率成像。本章介绍借助 CLEM 进行成像。本文提出的指导方针使研究人员能够分析细胞器的结构，特别是通过群体低分辨率成像捕获的罕见事件或通过活细胞成像捕获的瞬态事件，现在也可以通过电子显微镜进行高分辨率研究。
