Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore 138667, Singapore.
Biosens Bioelectron. 2012 Mar 15;33(1):293-8. doi: 10.1016/j.bios.2011.12.056. Epub 2012 Jan 4.
Enzyme-linked immunosorbent assays (ELISA) are commonly used for detecting cancer proteins at concentration in the range of about ng-μg/mL. Hence it often fails to detect tumor markers at the early stages of cancer and other diseases where the amount of protein is extremely low. Herein, we report a novel photonic crystal fiber (PCF) based surface enhanced Raman scattering (SERS) sensing platform for the ultrasensitive detection of cancer proteins in an extremely low sample volume. As a proof of concept, epidermal growth factor receptors (EGFRs) in a lysate solution from human epithelial carcinoma cells were immobilized into the hollow core PCF. Highly sensitive detection of protein was achieved using anti-EGFR antibody conjugated SERS nanotag. This SERS nanotag probe was realized by anchoring highly active Raman molecules onto the gold nanoparticles followed by bioconjugation. The proposed sensing method can detect low amount of proteins at ∼100 pg in a sample volume of ∼10 nL. Our approach may lead to the highly sensitive protein sensing methodology for the early detection of diseases.
酶联免疫吸附测定（ELISA）常用于检测浓度范围约为 ng-μg/mL 的癌症蛋白。因此，它常常无法在癌症和其他疾病的早期阶段检测到肿瘤标志物，因为这些疾病中蛋白质的含量极低。在此，我们报告了一种基于光子晶体光纤（PCF）的新型表面增强拉曼散射（SERS）传感平台，用于在极低的样品体积中对癌症蛋白进行超灵敏检测。作为概念验证，将人上皮癌细胞裂解液中的表皮生长因子受体（EGFR）固定到中空芯 PCF 中。使用与 EGFR 抗体偶联的 SERS 纳米标签实现了对蛋白质的高灵敏度检测。该 SERS 纳米标签探针通过将高活性拉曼分子锚定在金纳米粒子上，然后进行生物缀合来实现。所提出的传感方法可以在约 10 nL 的样品体积中检测到约 100 pg 的低量蛋白质。我们的方法可能会导致用于疾病早期检测的高灵敏度蛋白质传感方法。
