Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Leahurst Campus, Chester High Road, Neston, CH64 7TE, United Kingdom.
Vet Microbiol. 2012 Jun 15;157(3-4):391-7. doi: 10.1016/j.vetmic.2011.12.035. Epub 2012 Jan 8.
Risk of Campylobacter infection in humans has been associated with many sources, including dogs. C. upsaliensis is the most common species found in canines, and has been occasionally isolated from symptomatic humans. This study aimed to investigate the genetic diversity of 41 C. upsaliensis isolates carried by dogs and from nine isolates carried by humans using Multilocus sequence typing (MLST). We identified considerable genetic diversity amongst the C. upsaliensis isolates from both dogs and humans, identifying 45 different sequence types (STs). All STs were new, apart from that of the reference strain. Only three STs were found in more than one isolate: ST-72 (2 isolates), ST-98 (2 isolates) and ST-104 (3 isolates). ST-104 was the only ST to be encountered in both dogs and humans. Thirty-one of the 45 STs were assigned to one of 13 clonal complexes (CCs). Four of these CCs contained STs originating from both humans and dogs. None of the CCs contained exclusively human isolates, and two isolates from dogs within the same kennel belonged to the same CC. The large amount of diversity found in both dog and human isolates of C. upsaliensis, combined with the relatively small database, made it difficult to assign strains to sources of infection. This emphasizes the need to increase the size of the database. Dog and human isolates occasionally grouped together, however there were insufficient human-derived isolates to determine whether or not dogs are a common source of infection. Although C. upsaliensis infection is rare in humans, dogs still remain a potential source, and are therefore a possible zoonotic risk. Further work is needed to investigate the epidemiology of C. upsaliensis infection in humans.
人类感染弯曲菌的风险与许多来源有关，包括狗。弯曲杆菌属中最常见的物种是 C. upsaliensis，偶尔也会从有症状的人类中分离出来。本研究旨在使用多位点序列分型（MLST）调查 41 株来自犬和 9 株来自人类的 C. upsaliensis 分离株的遗传多样性。我们发现来自犬和人类的 C. upsaliensis 分离株具有相当大的遗传多样性，鉴定出 45 种不同的序列型（ST）。除参考菌株外，所有 ST 均为新发现。只有三种 ST 在一个以上的分离株中发现：ST-72（2 个分离株）、ST-98（2 个分离株）和 ST-104（3 个分离株）。ST-104 是唯一在犬和人类中均发现的 ST。45 个 ST 中有 31 个被分配到 13 个克隆复合体（CC）之一。其中 4 个 CC 包含来自人类和犬的 ST。没有一个 CC 仅包含人类分离株，同一犬舍的两个犬分离株属于同一 CC。在来自犬和人类的 C. upsaliensis 分离株中发现了大量的多样性，再加上相对较小的数据库，使得难以将菌株分配到感染源。这强调了需要增加数据库的大小。狗和人类的分离株偶尔会聚集在一起，但人类分离株的数量不足，无法确定狗是否是常见的感染源。虽然人类感染弯曲菌属的情况很少见，但狗仍然是一个潜在的来源，因此是一种潜在的人畜共患病风险。需要进一步研究人类弯曲菌属感染的流行病学。
